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Home DNA Modifying Enzymes and Cloning Technologies Products ET SSB

ET SSB
recombinant No

ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

  • Improve the processivity of DNA polymerase
  • Stabilization and marking of ssDNA structure
  • Increase the yield and specificitiy of PCR
  • Increase the yield and processivity of RT during RT-PCR
  • Improve DNA sequencing through regions with strong secondary structure
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Catalog # Concentration Size List Price Your Price Quantity
M2401S 500 µg/ml 50 µg $176.00
Please enter a quantity for at least one size
  • Product Information
    ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

    Figure 1: Improved multiplex PCR by ET SSB. Figure 1: improved multiplex PCR by ET SSB

    By adding ET SSB, specific amplification was accomplished in PCR using one to five primer pairs in incurring order (lanes 1-5). Lane 5C shows a PCR reaction using 5 primer pairs, with no ET SSB included (control reaction). Lane M: 100 bp DNA Ladder.
    Figure 2: Multiplex HDA was improved by ET SSB. Figure 2: Multiplex HDA was improved by ET SSB

    The HDA reactions were prepared with either single or two sets of primers to amplify the invE (A) and/or invA (B) from Salmonella typhimurium genomic DNA. Lane M: Low Molecular DNA Ladder; Lane A: invE gene; Lane B: invA gene; Lane AB: invE and invA genes with no ET SSB present; Lane AB**: invE and invA genes with ET SSB in the HDA reaction. ** the reaction contained ET SSB.

    Product Source

    An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism
    This product is related to the following categories:
    ssDNA Binding Proteins Products
    • Reagents Supplied

      Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration
    • Properties & Usage

      Unit Definition

      Sold by mass of pure protein as determined by OD280.

      Storage Buffer

      10 mM Tris-HCl
      100 mM KCl
      0.1 mM EDTA
      50% Glycerol
      pH 7.4 @ 25°C

      Heat Inactivation

      No

      Molecular Weight

      Apparent: 16 kDa
    • Product Notes
      1. ET SSB is active in any polymerase buffer. Add 200 ng of ET SSB per 50 µl reaction.
    • References
      1. Myers, T.W. and Romano, L.J. (1988). J. Biol. Chem. 263, 17006-17015.
      2. Reddy, M.S. (2000). Biochemistry. 39, 14250-14262.
      3. West, S.C., Cassuto, E. and Howard-Flanders, P. (1982). Mol. Gen. Genet. 186, 333-338.
      4. Goldmeyer, J., Kong, H., and Tang, W. (2007). J. Mol. Diagnostics. 9, 639-644.
      5. Delius, H., Mantell, N.J. and Alberts, B. (1972). J. Mol. Biol. 67, 341-350.
      6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1990). Nucl. Acids Res. 18, 1079.
      7. Chou, Q. (1992). Nucl. Acids Res. 20, 4371.
      8. Oshima, R.G. (1992). Biotechniques. 13, 188.
      9. Rapley, R. (1994). Mol. Biotechnol. 2, 295-298.
      10. Olszewski, M. et al. (2005). Mol. Cell Probes. 19, 203-205.
      11. Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
      12. Villalva, C. et al. (2001). Biotechniques. 31, 81-83, 86.
  • Citations & Technical Literature
  • Quality, Safety & Legal
    • Quality Control Assays
      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    • Specifications & Change Notifications

      Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
    • Certificate of Analysis
      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
    • Safety Data Sheets
      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
    • Legal and Disclaimers
      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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