This product is available in a glycerol-free format. Contact us for more information.
Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance
Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
Improved amplification reaction properties compared to Bst DNA Polymerase
Fast polymerization
Flexible reaction conditions including a higher salt tolerance and thermostability, as compared to wild-type Bst DNA Polymerase, Large Fragment (NEB #M0275)
WarmStart® version available for increased reaction efficiency and room temperature setup (NEB #M0538)
Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
LAMP Salt Tolerance
Performing LAMP reactions with increasing amounts of salt demonstrate increased resistance to inhibitors by using Bst 2.0. Bst 2.0 maintains high levels of activity where wild-type Bst DNA polymerase, large fragment, is completely inhibited.Rapid LAMP Detection
LAMP reactions amplifying different targets demonstrate a more rapid amplification threshold time using Bst 2.0 vs. Bst, large fragment.
Product Source
Bst 2.0 DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.
This product is related to the following categories:
Poole, C.B., Ettwiller, L., Tanner, N.A., Evans, T.C., Jr., Wanji, S.,Carlow, C.K. (2015) Genome filtering for new DNA biomarkers of Loa loa infection suitable for loop-mediated isothermal amplification. PLoS One; 10(9), e0139286.. PubMedID: 23272258, DOI: 10.1371/journal.pntd.0001948
Sanchita Bhadra, Yu Sherry Jiang, Mia R Kumar, Reed F Johnson, Lisa E Hensley, Andrew D Ellington (2015) Real-Time Sequence-Validated Loop-Mediated Isothermal Amplification Assays for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). PLoS One; 10, e0123126. PubMedID: 25856093, DOI: 10.1371/journal.pone.0123126
Publications
Poole CB, Zhang Y, Evans TC Jr., Carlow CK (2012) Diagnosis of brugian filariasis by loop-mediated isothermal amplification. PLoS Negl Trop Dis; 6(12), e1948.. PubMedID: 2327225, DOI: 10.1371/journal.pntd.0001948
Tanner NA, Zhang Y, Evans TC Jr. (2012) Simultaneous multiple target detection in real-time loop-mediated isothermal amplification Biotechniques; Aug, 81-9. PubMedID: 23030060, DOI: 10.2144/0000113902
Tanner NA, Zhang Y, Evans TC Jr (2015) Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques; Feb, 59-68. DOI: 10.2144/000114253
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
The purchase of NEB Bst products conveys to the purchaser the limited, nontransferable right to use the purchased products to perform loop-mediated isothermal amplification ("LAMP") for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd. and any use other than research may require a license from Eiken Chemical Co., Ltd. A patent is pending for NEB's Bst DNA Polymerase.
Trademarks
NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs Inc. WARMSTART™ is a trademark of New England Biolabs, Inc.
TRITON® is a registered trademark of Union Carbide Corporation. TWEEN® is a registered trademark of Uniqema Americas LLC.
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
Need a custom/large volume order? Contact us
Powered by Bioz
