New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

Q5U® Hot Start High-Fidelity DNA Polymerase

Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

  • Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
  • Prevent carryover contamination when combined with dUTP (NEB #N0459) and Uracil DNA Glycosylase (UDG) (NEB #M0372) treatment
  • Generate higher assembly efficiency and improve accuracy in USER® cloning
  • Enable room temperature setup with aptamer-based hot start formulation
  • Utilize optimized protocols for recommended applications




Catalog # Concentration Size List Price Your Price Quantity
M0515S 2,000 units/ml 100 units $167.00
M0515L 2,000 units/ml 500 units $647.00
Please enter a quantity for at least one size

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