Hot Start Taq 2X Master Mix
The industry standard with the hot start peformance
- Ideal for routine PCR applications
- Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
- 2X master mix format for easy reaction setup - just add template DNA and primers
Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb.
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activityrefrefref and a 5´ flap endonuclease activityrefref.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
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