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Epigenetics

Epigenetics is the study of heritable changes in gene expression not encoded in DNA. Changes in epigenetic states play vital roles in development and disease. Efficient approaches to decode the epigenome and epitranscriptome drive fundamental knowledge, diagnostics, and therapeutics. NEB has applied its expertise in enzymology to offer innovative approaches for epigenetic analyses that address challenges with conventional methods.

 

Tools for epigenetics discovery and development

Our epigenetics solutions simplify analyses of DNA methylation, hydroxymethylation, the epitranscriptome, chromatin accessibility, and chromatin interactions. Enzymatic methyl conversion enables superior mapping of DNA methylation or hydroxymethylation at single nucleotide resolution. NEBNext® library preparation workflows, and DNA polymerases optimized for GC-rich sequences, streamline next generation sequencing methylome analysis. EpiMark® enrichment kits efficiently profile genomes for relative abundance of methylated DNA or RNA. Liquid chromatography mass spectrometry analysis of nucleotide modifications is streamlined using our ribonucleases (RNases) and Nucleoside Digestion Mix. Sequence-specific digests with isoschizomer pairs offer simplified detection of DNA methylation at specific loci. Methylated DNA controls at specific sites can be generated with DNA methyltransferases. Chromatin interactions and accessibility can be explored in novel ways with next generation sequencing methods.

EpiMark® is a registered trademark of New England Biolabs, Inc.
EM-seq™ is a trademark of New England Biolabs, Inc.
Illumina® is a registered trademark of Illumina, Inc.

Choose Type:

Epigenetics includes these subcategories:
Chromatin Analysis
Epitranscriptome Analysis
DNA Methylation Analysis
Methylome Analysis
Methyltransferases for Epigenetics
FAQs for Epigenetics
Protocols for Epigenetics
Application Notes for Epigenetics
    Publications related to Epigenetics
    • Diep, D. and Zhang, K. (2011) Genome-wide mapping of the sixth base Genome Biol; 12, 116. PubMedID: 21682934, DOI: 10.1186/gb-2010-12-6-116
    • Nelson, F.K, Snyder, M., Gardner, A.F., Hendrickson, C.L., Shendure, J.A., Porreca, G.J., Church, G.M., Ausubel, F.M., Ju, J., Kieleczawa, J. and Slatko, B.E (2011) Introduction and historical overview of DNA sequencing Curr Protoc Mol Biol; Unit 7.0.1-7.0.18., PubMedID: 21987056
    • Wolff, E.M. et al. (2011) Hypomethylation of a LINE-1 Promoter Activates an Alternate Transcript of the MET Oncogene in Bladders with Cancer PLoS Genet; 6, 4:e1000917 . PubMedID: 20421991
    • Kinney, S. M., Chin, H. G., Vaisvila, R., Bitinaite, J., Zheng, Y., Estève, P. O., Feng, S., Stroud, H., Jacobsen, S. E., Pradhan, S. (2011) Tissue specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genome J Biol Chem; 286:28, 24685–2469. PubMedID: 21610077
    • Cohen-Karni, D, et al. (2011) The MspJI family of modification-dependent restriction endonucleases for epigenetic studies Proc Natl Acad Sci U S A; PubMedID: 21690366, DOI: 10.1073/pnas.1018448108
    • Stroud, H., et al. (2011) 5-Hydroxymethylcytosine is associated with enhancers and gene bodies in human embryonic stem cells Genome Biol; PubMedID: 21689397
    • Canc. Res. (2011) 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1 Cancer Res; 71, 1904-1911. PubMedID: 21239472
    • Ficz, G., et al. (2011) Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation Nature; PubMedID: 21460836, DOI: 10.1038/nature10008
    • Zhang, J. et al. (2011) Cyclophosphamide perturbs cytosine methylation in jurkat-T Cells through LSD1-mediated stabilization of DNMT1 Protein Chem Res Toxicol; PubMedID: 22007908
    • Wang, H., Guan, S., Quimby, A., Cohen-Karni, D., Pradhan, S., Wilson, G.G., Roberts, R.J., Zhu, Z.and Zheng, Y. (2011) Comparitive characterization of the PvuRts11 family and application in mapping genomic 5-hydroxymethylcytosine Nucleic Acids Res; PubMedID: 21813453, DOI: 10.1093/nar/gkr607
    • Sexton T, Kurukuti S, Mitchell JA, Umlauf D, Nagano T, Fraser P (2012) Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip Nat Protoc; 7(7), 1335-50. PubMedID: 22722369, DOI: 10.1038/nprot.2012.071
    • Ho, J.J., et al. (2012) Functional importance of Dicer protein in the adaptive cellular response to hypoxia J Biol Chem; 17, 29003-20. PubMedID: 22745131, DOI: 10.174/jbcM112.373365
    • Grant, T.J., et al. (2012) Antiproliferative small-molecule inhibitors of transcription factor LSF reveal oncogene addiction to LSF in hepatocellular carcinoma Proc Natl Acad Sci U S A; 109, 4503-4508. PubMedID: 22396589
    • Foraker, A.B., et al. (2012) Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG J Cell Biol; 198, 591-605.
    • Gu, L.Q., et al. (2012) Detection of miRNAs with a nanopore single-molecule counter Expert Rev Mol Diagn; 12, 573-584. PubMedID: 22845478
    • Gong, H. wt al. (2012) Near-infrared fluorescence imaging of mammalian cells and xenograft tumors with SNAP-tag PLoS One; 7, PubMedID: 22479502
    • Mooijman D, Dey S S, Boisset JC, Crosetto N, van Oudenaarden A (2016) Single-cell 5hmC sequencing reveals chromosome-wide cell-to-cell variability and enables lineage reconstruction Nat Biotechnol; 34, 852-857. PubMedID: 27347753, DOI: 10.1038/nbt.3598
    • Chernov AV., Reyes L., Peterson S., Strongin AY. (2015) Depletion of CG-Specific Methylation in Mycoplasma hyorhinis Genomic DNA after Host Cell Invasion PLoS One; 10, e0142529. PubMedID: 26544880, DOI: 10.1371/journal.pone.0142529
    • Kienhöfer S., Musheev M., Stapf U., Helm M., Schomacher L., Niehrs C., Schäfer A. (2015) GADD45a physically and functionally interacts with TET1Publication Differentiation; PubMedID: 26546041, DOI: 10.1016/j.diff.2015.10.003
    • Page A., Paoli P., Salvador E., White S., French J., Mann J. (2015) Hepatic Stellate Cell Transdifferentiation Involves Genome-Wide Remodeling of the DNA Methylation Landscape J Hepatol; PubMedID: 26632634, DOI: 10.1016/j.jhep.2015.11.024
    • Wee E., Ngo T., Trau M. (2015) A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation Sci Rep; 5, 15028. PubMedID: 26458746, DOI: 10.1038/srep15028
    • Wanunu, M. et al. (2010) Discrimination of methylcytosine from hydroxymethylcytosine in DNA molecular J Am Chem Soc; 133(3), PubMedID: 21155562
    • Laget, S., et al. (2010) The human proteins MBD5 and MBD6 associate with heterochromatin but they do not bind methylated DNA PLoS One; 5, PubMedID: 20700456
    • Wanunu, M., et al. (2010) Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors Nat Nanotechnol; 5, 807-814. PubMedID: 20972437
    • Zheng, Y. et al. (2010) A unique family of Mrr-like modification-dependent restriction endonucleases Nucleic Acids Res; 38(16), 5527-5534. PubMedID: 20444879
    • Jensen, H.M., et al. (2010) Engineering of a synthetic electron conduit in living cells Proc Natl Acad Sci U S A; 107, 19213-19218. PubMedID: 20956333
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • Epigenetics_Tutorial_TN

    Interactive Tutorial Explaining the Phenomenon of Epigenetics at a Molecular Level

    Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change.

  • Next-Generation-Solutions-for-Epigenetic-Analysis

    Next Generation Solutions for Epigenetic Analysis

    In this webinar hosted by Oxford Global, learn about the advantages of Enzymatic Methyl-seq (EM-seq®) and the epigenetic analyses such as cytosine methylation analysis of cell free DNA and intact long DNA fragments. This webinar also describes the development of NicE-seq (Nicking Enzyme assisted sequencing) a method for high-resolution open chromatin profiling of both native and FFPE samples.

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