Application Features

• High Sensitivity PCR
• High Throughput PCR
• Routine PCR
• AT-rich PCR
• Colony PCR
• Long PCR (up to ~6 kb genomic)

Storage Temperature

-20°C

1X Master Mix Composition

20 mM Tris-HCl
22 mM KCl
22 mM NH₄Cl
1.8 mM MgCl₂
5% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween® 20
0.2 mM dNTPs
1X Xylene Cyanol
1X Tartrazine
25 units/ml OneTaq Hot Start DNA Polymerase
pH 8.9@25°C

Heat Inactivation

No

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.

Companion Products

• Magnesium Chloride (MgCl₂) Solution
• OneTaq® DNA Polymerase
• OneTaq® Hot Start 2X Master Mix with GC Buffer
• OneTaq® Hot Start 2X Master Mix with Standard Buffer
• OneTaq® Hot Start DNA Polymerase
• OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

1. Product specifications for individual components in the OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer are available separately.
2. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C
3. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.

1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. How should I set up a PCR using the OneTaq® Hot Start Master Mixes?
2. Can I use my regular Taq-based cycling conditions for OneTaq® Hot Start DNA Polymerase based products?
3. How do I activate OneTaq® Hot Start Polymerase?
4. What are the stability and storage requirements of the Quick-Load® OneTaq® Master Mixes?
5. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
6. How long a product can be made by OneTaq® DNA Polymerase?
7. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
8. What is the fidelity of OneTaq® DNA Polymerase?
9. Can OneTaq® DNA Polymerase be used in colony PCR?
10. Where can I find help troubleshooting my PCR?
11. How should I determine an appropriate annealing temperature for my reaction?
12. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

1. Protocol for OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer (M0488)

• DNA Polymerase Selection Chart
• PCR Selection Chart
• Thermophilic DNA Polymerases

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• PCR Troubleshooting Guide

• NEBioCalculator
• PCR Selector
• Tm Calculator

• Robust Colony PCR from Multiple E coli Strains using OneTaq QuickLoad Master Mixes M0488

Quality Control Assays

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0488S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0488S_L_v1_0261706

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Notice to Purchaser:
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based OneTaq™ Hot Start Quick-Load 2X Master Mix with Standard Buffer for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based OneTaq™ Hot Start Quick-Load 2X Master Mix with Standard Buffer requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.

Trademarks

NEW ENGLAND BIOLABS^®, ONETAQ^®, QUICK-LOAD^® and THERMOPOL^® are registered trademarks of New England Biolabs, Inc.
DEEP VENT^™ is a trademark of New England Biolabs, INC.

IGEPAL^® is a registered trademark of Rhodia Operations.
TWEEN^® is a registered trademark of Uniqema Americas LLC.

• This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.