OneTaq® Hot Start 2X Master Mix with GC Buffer
The ONE polymerase for your endpoint PCR needs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- Obtain high yields with GC rich templates (>65%)
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
How to Amplify GC-rich DNA
5 Tips for Setting Up Your PCR
Why is Tm Important in Primer Design?
Overview of PCR
OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep Vent ™ DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® High GC Enhancer -20 50 %
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