Reaction Conditions

IX Cas9 Nuclease Reaction Buffer
Incubate at 37°C

1X Cas9 Nuclease Reaction Buffer
100 mM NaCl
5 mM MgCl₂
20 mM HEPES
0.1 mM EDTA
(pH 6.5 @ 25°C)

Storage Temperature

-20°C

Storage Conditions

0.1 mM EDTA
50% Glycerol
1 mM DTT
10 mM Tris-HCl
300 mM NaCl
pH 7.4 @ 25°C

Heat Inactivation

65°C for 5 min

Companion Products

• EnGen® Mutation Detection Kit
• EnGen® sgRNA Synthesis Kit, S. pyogenes

1. 1,000 nM is equal to 159 ng/μl.

1. Jinek M. et al. (2012). Science. 816-821 doi: 10.1126/Science.1225829. Epub 2012 Jun 28.. PubMedID: 22745249,

1. Why do I observe incomplete digestion?
2. Why does digestion efficiency differ between two SgRNAs?
3. Does NEB provide plasmids for gRNA cloning?
4. Does Cas9 Nuclease contain a nuclear localization signal (NLS)? 
5. Is Cas9 Nuclease fused with a N-terminal 6XHis tag?

1. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
2. Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (NEB #M0386)

• CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology

• NEBioCalculator

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Cas9 Nuclease, S. pyogenes
• Cas9 Nuclease Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Construction of an sgRNA Cas9 expression vector

CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology