- Removal of ss-oligonucleotide primers
- Removal of terminal phosphorothioated ss-oligonucleotide primers
- Mapping positions of introns in genomic DNA
- Removal of single-stranded DNA from dsDNA
Exonuclease VII, (Exo VII) derived from E. coli, cleaves single-stranded DNA (ssDNA) from both 5´→3´ and 3´→5´ direction. This enzyme is not active on linear or circular dsDNA (1,2). It is useful for removal of single stranded oligonucleotide primers (3) from a completed PCR reaction when different primers are required for subsequent PCR reactions. Digestion of ssDNA by Exonuclease VII is metal-independent.
Product SourceAn E. coli strain that carries cloned Exonuclease VII (XseA and XseB) genes from E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration Exonuclease VII Reaction Buffer -20 5X
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