Exonuclease VII

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0379S     -20       Exonuclease VII M0379SVIAL -20 1 x 0.02 ml 10,000 units/ml   Exonuclease VII Reaction Buffer B0379SVIAL -20 1 x 1.5 ml 5 X
M0379L     -20       Exonuclease VII M0379LVIAL -20 1 x 0.1 ml 10,000 units/ml   Exonuclease VII Reaction Buffer B0379SVIAL -20 1 x 1.5 ml 5 X

Application Features

• Removal of single-stranded oligonucleotide primers after PCR (3)
• Removal of terminal phosphorothioated ss-oligonucleotide primers after PCR
• Mapping positions of introns in genomic DNA (4)
• Removal of single-stranded DNA from dsDNA

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Chase, et al. (1974). J. Biol. Chem. 249, 4545-4552.
2. Chase, et al. (1974). J. Biol. Chem. 249, 4553-4561.
3. Li, H. et al. (1991). Nucl. Acids Res. 19, 3139-3141.
4. Berk, A.J. et al. (1978). Proc. Natl. Acad. Sci. USA. 75, 1274-1278.

1. Protocol for Exonuclease VII (M0379)

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• NEB Diluent and Buffer Table
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

• Choosing the Right Exonuclease (2019)

1. What is the activity of Exonuclease VII in the following buffers?
2. Will Exonuclease VII work in a PCR buffer?
3. What is the molecular weight of the Exonuclease VII?
4. Is Exonuclease VII a tagged protein?
5. Does Exonuclease VII cut RNA?
6. Can DNA be blunted using Exonuclease VII?
7. What is the difference between Exonuclease VII and Exonuclease I (NEB# M0293)?
8. How to remove primers from a PCR reaction?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0379S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0379L_v1_10011606
• M0379S_v1_10022780
• M0379S_v1_10041617
• M0379S_v1_10045753
• M0379L_v1_10050888
• M0379L_v1_10052213
• M0379L_v1_10054777
• M0379S_v1_10052214
• M0379L_v1_10056006
• M0379S_v1_10056007
• M0379L_v1_10062309
• M0379L_v1_10080131
• M0379S_v1_10080133
• M0379L_v1_10095227
• M0379S_v1_10111551
• M0379S_v1_10129206
• M0379L_v1_10129204
• M0379S_v1_10153916
• M0379L_v1_10187595
• M0379S_v1_10188454
• M0379S_L_v1_0021710
• M0379S_L_v1_0031804

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Exonuclease VII
• Exonuclease VII Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.