Human single-strand-selective monofunctional uracil-DNA Glycosylase SMUG1 excises deoxyuracil and deoxyuracil-derivatives bearing an oxidized group at C5, such as 5-hydroxyuracil, 5-hydroxymethyluracil and 5-formyluracil in ssDNA and dsDNA (1,2,3).
- Oxidative DNA damage studies
- Single cell gel electrophoresis (comet assay) (4,5,6)
Product SourceAn E. coli strain which carries the cloned human SMUG1 gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 1 -20 10 X Purified BSA -20 10 mg/ml
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