T7 DNA Polymerase (unmodified)
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The high polymerization rate of the enzyme makes long incubations unnecessary.
- Second strand synthesis in site-directed mutagenesis protocols
- Gap filling reaction (no strand displacement)
- T7 DNA Polymerase is not suitable for DNA sequencing
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3´→ 5´ exonuclease (1,2,3). The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.
Product SourceT7 DNA Polymerase consists of two subunits: T7 gene 5 protein (84 kilodaltons) and E.coli thioredoxin (12 kilodaltons) (1,4-7). Each protein is cloned and overexpressed in a T7 expression system in E. coli (4).
The following reagents are supplied with this product:
Store at (°C) Concentration T7 DNA Polymerase Reaction Buffer -20 10X BSA, Molecular Biology Grade -20 20 mg/ml
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