Mung Bean Nuclease

Description

A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends.

Highlights

  • Removal of single-stranded extensions (3' and 5') to leave ligatable blunt ends
  • Transcriptional mapping
  • Cleavage of hairpin loops
  • Supplied with 10X Reaction Buffer

Product Source

Mung bean sprouts.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Mung Bean Nuclease Reaction Buffer10X

Advantages and Features

Applications

  • Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
  • Transcriptional mapping
  • Cleavage of hairpin loops
  • Excision of gene coding sequences from genomic DNA
  • Generation of new restriction sites

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble total nucleotide in a total reaction volume of 50 μl in 1 minute at 37°C in 1X Mung Bean Nuclease Reaction Buffer with 0.5 mg/ml denatured Calf Thymus DNA.

Reaction Conditions

1X Mung Bean Nuclease Reaction Buffer
Incubate at 30°C

1X Mung Bean Nuclease Reaction Buffer:
30 mM NaCl
50 mM sodium acetate
1 mM ZnSO4
pH 5 @ 25°C

Storage Conditions

10 mM sodium acetate
0.1 mM Zinc Acetate
1 mM Cysteine
50% Glycerol
0.001% Triton® X-100
pH 5.0 @ 25°C

Molecular Weight

Theoretical: 39 kDa

Notes

  1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn2+. The zinc acetate in the storage buffer fulfills the Zn2+ requirement of the enzyme even after dilution in a reaction.

References

  1. Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
  2. McCutchan, T.F. et al. (1984). Science. 225, 626-628.

FAQs

  1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?
  2. Is the Mung Bean Nuclease active in other NEBuffers?
  3. Can Mung Bean Nuclease be heat inactivated?
  4. Does Zn2+ need to be added to a Mung Bean Nuclease reaction?
  5. Will Mung Bean Nuclease degrade double stranded DNA?

Protocols

  1. Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • Purified free of double-strand exonuclease contamination.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.