Mung Bean Nuclease

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0250S     -20       Mung Bean Nuclease M0250SVIAL -20 1 x 0.15 ml 10,000 units/ml   Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X
M0250L     -20       Mung Bean Nuclease M0250LVIAL -20 1 x 0.75 ml 10,000 units/ml   Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X

Application Features

• Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
• Transcriptional mapping
• Cleavage of hairpin loops
• Excision of gene coding sequences from genomic DNA
• Generation of new restriction sites

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Mung Bean Nuclease Reaction Buffer

1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn²⁺. The zinc acetate in the storage buffer fulfills the Zn²⁺ requirement of the enzyme even after dilution in a reaction.
2. Do not attempt to heat inactivate, DNA will “breathe” before enzyme inactivates, causing undesirable degradation. 

1. Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
2. McCutchan, T.F. et al. (1984). Science. 225, 626-628.

1. Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250)

• Traditional Cloning Quick Guide

• Activities of Exonucleases and Non-specific Endonucleases
• Blunting Selection Chart
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector
• NEBcloner®

1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?
2. Is the Mung Bean Nuclease active in other NEBuffers?
3. Can Mung Bean Nuclease be heat inactivated?
4. Does Zn²⁺ need to be added to a Mung Bean Nuclease reaction?
5. Will Mung Bean Nuclease degrade double stranded DNA?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0250S_L_v1
• M0250S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0250S_v1_10018062
• M0250L_v1_10022243
• M0250S_v1_10035014
• M0250L_v1_10035015
• M0250S_v1_10035019
• M0250L_v1_10044911
• M0250S_v1_10054611
• M0250L_v1_10054610
• M0250S_v1_10070946
• M0250S_v1_10089432
• M0250S_v1_10103409
• M0250S_v1_10106726
• M0250L_v1_10089435
• M0250L_v1_10130304
• M0250S_v1_10130305
• M0250S_v1_10143533
• M0250S_v2_10149098
• M0250L_v2_10157968

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Mung Bean Nuclease
• Mung Bean Nuclease Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.