FAQ: Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?

Mung Bean Nuclease must be used if the goal is to chew back a 5' extension. DNA Polymerase I, Large (Klenow) Fragment will fill in a 5' extension and it will chew back a 3' extension. Mung Bean Nuclease can be used to chew back a 3' extension, but DNA Polymerase I, Large (Klenow) Fragment may be a slightly better choice due to Mung Bean Nuclease's ability to act at nicks and degrade ends as they "breathe". Note: A 3' overhang can not be filled in.
  1. DNABluntingVideo_thumb

    DNA Blunting Tutorial

    The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.

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    What are exonucleases and their applications?

    Learn about exonucleases and some of their common applications.