RNase If eliminates single-stranded RNA from DNA and protein preparations
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of DNA endonucleases and exonucleases
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Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Product SourceAn E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 3 -20 10 X
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