- Single-cell gel electrophoresis (Comet Assay)
- Alkaline elution
- Alkaline unwinding
- Isolation from a recombinant source
- Supplied with 10X Reaction Buffer
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Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3´ and 5´ to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil (1,2).
Product SourceAn E.coli strain that carries the cloned fpg gene (3)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 1 -20 10 X Purified BSA -20 10 mg/ml
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