EcoRI Methyltransferase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0211S     -20       EcoRI Methyltransferase M0211SVIAL -20 1 x 0.25 ml 40,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Materials Sold Separately

• S-adenosylmethionine (SAM)

1. EcoRI Methyltransferase is inhibited by MgCl₂. Only 50% activity is retained at a concentration of 4 mM MgCl₂.

1. Hoffman, J.L. (1986). biochemistry. 25, 4444-4449.

1. Recommended Protocol for ³H labeling of DNA
2. Recommended Protocol for Methylation of Genomic DNA

• Dam and Dcm Methylases of E. coli

1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
2. What should be considered if the methylation is not going to completion?
3. Can EcoRI Methyltransferase be heat inactivated?
4. Does EcoRI Methyltransferase require MgCl₂?
5. Will EcoRI methylation block the ApoI restriction enzyme?
6. Will any NEB methyltransferases (methylases) work on RNA?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0211S_v1
• M0211S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0211S_v1_10020383
• M0211S_v1_10038542
• M0211S_v1_10044277
• M0211S_v1_10047791
• M0211S_v1_10054602
• M0211S_v1_10056505
• M0211S_v1_10093129
• M0211S_v2_10121432
• M0211S_v2_10165493
• M0211S_v2_10175645
• M0211S_v2_10183231

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• EcoRI Methyltransferase
• rCutSmart™ Buffer
• S-adenosylmethionine (SAM)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.