Exonuclease III (E. coli)
- 3' → 5' exonuclease
- Production of unidirectional nested deletions
- Site-directed mutagenesis
- Preparation of strand-specific probes
- Preparation of single-stranded substrates for dideoxy sequencing
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
Catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA (1). A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules (2).
The preferred substrates are blunt or recessed 3´-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3´-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage.
Exonuclease III activity depends partially on helical structure (4) and displays sequence dependence (C>A=T>G; ref. 5). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Exonuclease III has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities (1).
Product SourcePurified from E.coli K-12, BE257/pSGR3 strain (kindly supplied by B. Weiss)
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 1 -20 10 X
- Product Categories:
- Nucleases Products
- Polymerases for DNA Manipulation
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.