E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4° C – 37° C).
- Specific Activity: 6,000 units/mg
- Supplied with 10X Reaction Buffer
Product SourcePurified from E. coli strain containing a cloned E. coli DNA Ligase gene.
The following reagents are supplied with this product:
Store at (°C) Concentration E. coli DNA Ligase Reaction Buffer -20 10X
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