glycobiology banne


New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

To find all of our Glycobiology Application Notes, click here.

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Glycobiology includes these subcategories:
FAQs for Glycobiology
Application Notes for Glycobiology
    Publications related to Glycobiology
    • Stech M, Quast RB, Sachse R, Schulze C, Wüstenhagen DA, Kubick S (2014) A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells PLoS One; 9(5), e96635. PubMedID: 24804975, DOI: 10.1371/journal.pone.0096635
    • Wicht O, Burkard C, de Haan CA, van Kuppeveld FJ, Rottier PJ, Bosch BJ (2014) Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell J Virol; 88(9), 4943-52. PubMedID: 24554652, DOI: 10.1128/JVI.03451-13
    • Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE (2014) Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris Appl Environ Microbiol; 80(9), 2746-53. PubMedID: 24561590, DOI: 10.1128/AEM.03914-13
    • Magnelli PE, Bielik AM, Guthrie EP. (2011) Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases J Vis Exp; 58 , pii: 3749 . PubMedID: 21987255, DOI: 10.3791/3749
    • Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D. (2005) Analysis and function of prototype foamy virus envelope N glycosylation J Virol; 79 , 7664-72 . PubMedID: 15919919
    • Srikrishna, G., Brive, L., Freeze, H.H. (2005) Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem Biophys Res Commun; 332 , 1020-7 . PubMedID: 15922305
    • Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B. (2005) N-Glycans of Caenorhabditis elegans are specific to developmental stages J Biol Chem;  280 , 26063-72 . PubMedID: 15899899
    • O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris (2017) Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex J Proteome Res; 16, 4237-4243.
    • Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. Anal Chem.. PubMedID: 27033327
    • Kalidas, C., Joshi, L., Batt, C. (2001) Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng; 14 , 201-7 . PubMedID: 11342718
    • Möykkynen T, Coleman SK, Semenov A, Keinänen K (2014) The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization J Biol Chem; 289(19), 13197-205. PubMedID: 24652293, DOI: 10.1074/jbc.M113.526301
    • Kwon HM, Lee KH, Han BW, Han MR, Kim DH, Kim DE (2014) An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells PLoS One; 9(5), e97574. PubMedID: 24835440, DOI: 10.1371/journal.pone.0097574
    • Wright CR, Brown EL, Della-Gatta PA, Ward AC, Lynch GS, Russell AP (2014) G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle Front Physiol; 5, 170. PubMedID: 24822049, DOI: 10.3389/fphys.2014.00170
    • Itahana Y, Han R, Barbier S, Lei Z, Rozen S, Itahana K (2014) The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense Oncogene; PubMedID: 24858040, DOI: 10.1038/onc.2014.119
    • Botto L, Cunati D, Coco S, Sesana S, Bulbarelli A, Biasini E, Colombo L, Negro A, Chiesa R, Masserini M, Palestini P (2014) Role of lipid rafts and GM1 in the segregation and processing of prion protein PLoS One; 9(5), e98344. PubMedID: 24859148, DOI: 10.1371/journal.pone.0098344
    • Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis J Biol Chem; 289(19), 13347-61. PubMedID: 24668812, DOI: 10.1074/jbc.M113.543710
    • Haller G, Li P, Esch C, Hsu S, Goate AM, Steinbach JH (2014) Functional characterization improves associations between rare non-synonymous variants in CHRNB4 and smoking behavior PLoS One; 9(5), e96753. PubMedID: 24804708, DOI: 10.1371/journal.pone.0096753
    • Arakel EC, Brandenburg S, Uchida K, Zhang H, Lin YW, Kohl T, Schrul B, Sulkin MS, Efimov IR, Nichols CG, Lehnart SE, Schwappach B (2014) Tuning the electrical properties of the heart by differential trafficking of KATP ion channel complexes J Cell Sci; 127(Pt 9), 2106-19. PubMedID: 24569881, DOI: 10.1242/jcs.141440
    • Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ (2014) Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster PLoS Genet; 10(5), e1004349. PubMedID: 24785692, DOI: 10.1371/journal.pgen.1004349
    • Wong-Madden, S.T., Landry, D., and Guthrie, E.P. (1997) Discovery and Uses of Novel Glycosidases Techniques in Glycobiology; 401-408 .
    • Eichler, R., Lenz, O., Garten, W., Strecker, T. (2006) The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol J;  3 , 41 . PubMedID: 16737539
    • Sielaff I. et al. (2006) Protein function microarrays based on self-immobilizing and self-labeling fusion proteins Chembiochem; 7 , 194-202 . PubMedID: 16342318
    • O’Reilly, M.K., Zhang, G., Imperiali, B. (2006) In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry; 8 , 9593-603 . PubMedID: 16878994
    • Hagen, B.M., Sanders, K.M. (2006) Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am J Physiol Cell Physiol; 1 , 750-6 . PubMedID: 16738006
    • Velho, A.M., Jarvis, S.M. (2009) Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp Cell Res; 315 , 2312-21 . PubMedID: 19379732
    • Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. (2009) Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res; 337 , 79-89 . PubMedID: 19415334
    • Lee, K.J., Jung, J.H., Lee, J.M,, So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380 , 223-9 . PubMedID: 19167352
    • Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. (2009) Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol Biol; 534 , 213-23 .
    • Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. (2009) Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjugate Chem; 20 , 1228-36 . PubMedID: 19425533
    • Piro, J.R., Harris, B.T., Nishina, K., Soto, C., Morales, R., Rees, J.R., Supattapone, S. (2009) Prion protein glycosylation is not required for strain-specific neurotropism J Virol; 83 , 5321-8 .
    • Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. (2009) A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology; 57 , 287-94 . PubMedID: 19500602
    • Lee, K.J., Jung, J.H., Lee, J.M., So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. (2009) High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem Biophys Res Commun; 380 , 223-9 . PubMedID: 19167352
    • Teuton, J.R., Brandt, C.R. (2007) Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells J Virol; 81 , 3731-9 . PubMedID: 17229687
    • Song, Y., Choi, M.H., Park, J.N., Kim, M.W., Kim, E.J., Kang, H.A., Kim, J.Y. (2007) Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans Appl Environ Microbiol; 73 , 4446-54 . PubMedID: 17513593
    • Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., Tiemeyer, M. (2007) Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo J Biol Chem; 282 , 9127-42 . PubMedID: 17264077
    • Oh, D.B., Park, J.S., Kim, M.W., Cheon, S.A., Kim, E.J., Moon, H.Y., Kwon, O., Rhee, S.K., Kang, H.A. (2008) Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly Biotechnol J; 3 , 659-68 . PubMedID: 18320567
    • Macnaughtan, M.A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., Moremen, K.W., Prestegard, J.H. (2008) 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I J Am Chem Soc; 130 , 11864-5 . PubMedID: 18700760
    • Park, Y., Zhang, Z., Laremore, T.N., Li, B., Sim, J.S., Im, A.R., Ahn, M.Y., Kim, Y.S., Linhardt, R.J., Glycoconj, J. (2008) Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica) Glycoconj J; 25 , 863-77 . PubMedID: 18670878
    • Fujita, N., Tamura, A., Higashidani, A., Tonozuka, T., Freeze, H.H., Nishikawa, A. (2008) The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells FEBS J; 275 , 788-98 . PubMedID: 18215164
    • Zhuo, Y., Chammas, R., Bellis, S.L. (2008) Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis J Biol Chem; 283 , 22177-85 . PubMedID: 18676377
    • Wagner-Rousset, E., Bednarczyk, A., Bussat, M.C., Colas, O., Corvaïa, N., Schaeffer, C., Van Dorsselaer, A., Beck, A. (2008) The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies J Chromatogr B Analyt Technol Biomed Life Sci; 872, 23-37. PubMedID: 18672411
    • Graham, D.R., Mitsak, M.J., Elliott, S.T., Chen, D., Whelan, S.A., Hart, G.W., Van Eyk, J.E. (2008) Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses Proteomics; 872, 23-37. PubMedID: 19072736
    • Banala J. et al. (2008) Caged substrates for protein labeling and immobilization Chembiochem; 4 , 38-41 . PubMedID: 18033718
    • Baudino L, Shinohara Y, Nimmerjahn F, Furukawa J, Nakata M, Martínez-Soria E, Petry F, Ravetch JV, Nishimura S, Izui S. (2008) Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions J Immunol; 181(9) , 6664-9 . PubMedID: 18941257
    • Magnelli P, Bielik A, Guthrie E.  (2012) Identification and characterization of protein glycosylation using specific endo- and exoglycosidases Methods Mol Biol; 801 , 189-211 . PubMedID: 21987255
    • Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M. (2002) Modulation of postendocytic sorting of G protein-coupled receptors Science; 297 , 615-20 . PubMedID: 12142540
    • Vainauskas, S., Kirk, C.H., Petralia, L., Guthrie, E.P., McLeod, E., Bielik, A., Luebbers, A., Foster, J.M., Hokke, C.H., Rudd, P.M., Shi, X., Taron, C.H (2018) A novel broad specificity fucosidase capable of core a1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes Sci Rep; PubMedID: 29934601, DOI: 1038/s41598-018-27797-0
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