glycobiology banne

Glycobiology

New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.

To find all of our Glycobiology Application Notes, click here.

Glycobiology includes these subcategories:

Endoglycosidases
Exoglycosidases
Heparinases

FAQs for Glycobiology

    Publications related to Glycobiology

  1. Kalidas, C., Joshi, L., Batt, C. 2001. Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. . 14 , PubMedID: 11342718, DOI:
  2. Magnelli PE, Bielik AM, Guthrie EP. 2011. Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases J Vis Exp. . 58 , PubMedID: 21987255, DOI: 10.3791/3749
  3. Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M. 2002. Modulation of postendocytic sorting of G protein-coupled receptors Science . 297 , PubMedID: 12142540, DOI:
  4. Sielaff I. et al. 2006. Protein function microarrays based on self-immobilizing and self-labeling fusion proteins ChemBioChem. . 7 , PubMedID: 16342318, DOI:
  5. Eichler, R., Lenz, O., Garten, W., Strecker, T. 2006. The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol. J. .  3 , PubMedID: 16737539, DOI:
  6. O’Reilly, M.K., Zhang, G., Imperiali, B. 2006. In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry . 8 , PubMedID: 16878994, DOI:
  7. Hagen, B.M., Sanders, K.M. 2006. Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am. J. Physiol.Cell. Physiol.  . 1 , PubMedID: 16738006, DOI:
  8. Stech M, Quast RB, Sachse R, Schulze C, Wüstenhagen DA, Kubick S 2014. A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells PLoS One. 9(5), PubMedID: 24804975, DOI: 10.1371/journal.pone.0096635
  9. Kwon HM, Lee KH, Han BW, Han MR, Kim DH, Kim DE 2014. An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells PLoS One. 9(5), PubMedID: 24835440, DOI: 10.1371/journal.pone.0097574
  10. Botto L, Cunati D, Coco S, Sesana S, Bulbarelli A, Biasini E, Colombo L, Negro A, Chiesa R, Masserini M, Palestini P 2014. Role of lipid rafts and GM1 in the segregation and processing of prion protein PLoS One. 9(5), PubMedID: 24859148, DOI: 10.1371/journal.pone.0098344
  11. Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ 2014. Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster PLoS Genet. 10(5), PubMedID: 24785692, DOI: 10.1371/journal.pgen.1004349
  12. Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA 2014. Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis J Biol Chem. 289(19), PubMedID: 24668812, DOI: 10.1074/jbc.M113.543710
  13. Möykkynen T, Coleman SK, Semenov A, Keinänen K 2014. The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization J Biol Chem. 289(19), PubMedID: 24652293, DOI: 10.1074/jbc.M113.526301
  14. Arakel EC, Brandenburg S, Uchida K, Zhang H, Lin YW, Kohl T, Schrul B, Sulkin MS, Efimov IR, Nichols CG, Lehnart SE, Schwappach B 2014. Tuning the electrical properties of the heart by differential trafficking of KATP ion channel complexes J Cell Sci. 127(Pt 9), PubMedID: 24569881, DOI: 10.1242/jcs.141440
  15. Itahana Y, Han R, Barbier S, Lei Z, Rozen S, Itahana K 2014. The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense Oncogene. , PubMedID: 24858040, DOI: 10.1038/onc.2014.119
  16. Wicht O, Burkard C, de Haan CA, van Kuppeveld FJ, Rottier PJ, Bosch BJ 2014. Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell J Virol. 88(9), PubMedID: 24554652, DOI: 10.1128/JVI.03451-13
  17. Wright CR, Brown EL, Della-Gatta PA, Ward AC, Lynch GS, Russell AP 2014. G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle Front Physiol. 5, PubMedID: 24822049, DOI: 10.3389/fphys.2014.00170
  18. Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE 2014. Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris Appl Environ Microbiol. 80(9), PubMedID: 24561590, DOI: 10.1128/AEM.03914-13
  19. Haller G, Li P, Esch C, Hsu S, Goate AM, Steinbach JH 2014. Functional characterization improves associations between rare non-synonymous variants in CHRNB4 and smoking behavior PLoS One. 9(5), PubMedID: 24804708, DOI: 10.1371/journal.pone.0096753
  20. Srikrishna, G., Brive, L., Freeze, H.H. 2005. Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem. Biophys. Res. Commun. . 332 , PubMedID: 15922305, DOI:
  21. Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B. 2005. N-Glycans of Caenorhabditis elegans are specific to developmental stages J. Biol. Chem. .  280 , PubMedID: 15899899, DOI:
  22. Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D. 2005. Analysis and function of prototype foamy virus envelope N glycosylation J. Virol.  . 79 , PubMedID: 15919919, DOI:
  23. Wong-Madden, S.T., Landry, D., and Guthrie, E.P. 1997. Discovery and Uses of Novel Glycosidases Techniques in Glycobiology. , PubMedID: , DOI:
  24. Magnelli P, Bielik A, Guthrie E.  2012. Identification and characterization of protein glycosylation using specific endo- and exoglycosidases Methods Mol Biol. . 801 , PubMedID: 21987255, DOI:
  25. Teuton, J.R., Brandt, C.R. 2007. Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells  J. Virol. . 81 , PubMedID: 17229687, DOI:
  26. Song, Y., Choi, M.H., Park, J.N., Kim, M.W., Kim, E.J., Kang, H.A., Kim, J.Y. 2007. Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans Appl. Environ. Microbiol.  . 73 , PubMedID: 17513593, DOI:
  27. Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., Tiemeyer, M. 2007. Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo J. Biol. Chem. . 282 , PubMedID: 17264077, DOI:
  28. Piro, J.R., Harris, B.T., Nishina, K., Soto, C., Morales, R., Rees, J.R., Supattapone, S. 2009. Prion protein glycosylation is not required for strain-specific neurotropism  J Virol.  . 83 , PubMedID: , DOI:
  29. Velho, A.M., Jarvis, S.M. 2009. Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp. Cell Res. . 315 , PubMedID: 19379732, DOI:
  30. Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. 2009. Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjug. Chem. . 20 , PubMedID: 19425533, DOI:
  31. Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. 2009. Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol. Biol. . 534 , PubMedID: , DOI:
  32. Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. 2009. A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology . 57 , PubMedID: 19500602, DOI:
  33. Lee, K.J., Jung, J.H., Lee, J.M., So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. 2009. High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem. Biophys. Res. Commun. . 380 , PubMedID: 19167352, DOI:
  34. Lee, K.J., Jung, J.H., Lee, J.M,, So, Y., Kwon, O., Callewaert, N., Kang, H.A., Ko, K., Oh, D.B. 2009. High-throughput quantitative analysis of plant N-glycan using a DNA sequencer Biochem. Biophys. Res. Commun.  . 380 , PubMedID: 19167352, DOI:
  35. Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. 2009. Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res. . 337 , PubMedID: 19415334, DOI:
  36. Macnaughtan, M.A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., Moremen, K.W., Prestegard, J.H. 2008. 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I J. Am. Chem Soc. . 130 , PubMedID: 18700760, DOI:
  37. Zhuo, Y., Chammas, R., Bellis, S.L. 2008. Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis J. Biol. Chem. . 283 , PubMedID: 18676377, DOI:
  38. Fujita, N., Tamura, A., Higashidani, A., Tonozuka, T., Freeze, H.H., Nishikawa, A. 2008. The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells FEBS J.  . 275 , PubMedID: 18215164, DOI:
  39. Wagner-Rousset, E., Bednarczyk, A., Bussat, M.C., Colas, O., Corvaïa, N., Schaeffer, C., Van Dorsselaer, A., Beck, A. 2008. The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.. 872, PubMedID: 18672411, DOI:
  40. Graham, D.R., Mitsak, M.J., Elliott, S.T., Chen, D., Whelan, S.A., Hart, G.W., Van Eyk, J.E. 2008. Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses Proteomics. 872, PubMedID: 19072736, DOI:
  41. Park, Y., Zhang, Z., Laremore, T.N., Li, B., Sim, J.S., Im, A.R., Ahn, M.Y., Kim, Y.S., Linhardt, R.J., Glycoconj, J. 2008. Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica) Glycoconjugate Journal. 25 , PubMedID: 18670878, DOI:
  42. Banala J. et al. 2008. Caged substrates for protein labeling and immobilization Chembiochem . 4 , PubMedID: 18033718, DOI:
  43. Baudino L, Shinohara Y, Nimmerjahn F, Furukawa J, Nakata M, Martínez-Soria E, Petry F, Ravetch JV, Nishimura S, Izui S. 2008. Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions J Immunol. . 181(9) , PubMedID: 18941257, DOI:
  44. Oh, D.B., Park, J.S., Kim, M.W., Cheon, S.A., Kim, E.J., Moon, H.Y., Kwon, O., Rhee, S.K., Kang, H.A. 2008. Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly Biotechnol. J. . 3 , PubMedID: 18320567, DOI:

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. Glycobiology_Animation_thumb

    Overview of Glycobiology

    Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation.

  2. $name

    Identification and Characterization of Protein Glycosylation P6039S

    Learn how glycosidases are used to analyze multiple glycoprotein samples. Here, the model glycoprotein, hCGβ, which carries both N- and O-glycans, is demonstrated.

  3. NEBTV_An-engineered-high-affinity-Fbs1-carbohydrate-binding-protein-for-selective-capture-of-N-glycans-and-N-glycopeptides_720

    Behind the Paper: An engineered Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides

    Minyong and Jim summarize their recent Nature Communications publication describing selective capture of N-glycans and N-glycopeptides by an engineered high affinity Fbs1 carbohydrate binding protein.

  4. Heparin_Complexity_thumb

    Elucidating the Complexity of Heparin Oligosaccharide Analysis

    Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis.