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  • Amylose Magnetic Beads

    Description

    An affinity matrix for the small-scale isolation and purification of MBP-fusion proteins. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range. This permits the isolation of MBP-fusion proteins from cell culture supernatants. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that may interact with the immobilized fusion protein.

    Supplied as a 10 mg/ml suspension in water containing 20% ethanol.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Properties and Usage

    Storage Temperature

    4°C

    Storage Conditions

    20% Ethanol

    Binding Capacity

    10 ug MBP5* paromysin Δ sal / mg amylose magnetic beads

    Storage Notes

    • Do not freeze

    Shipping Notes

    • 4°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Isolation of MBP-fusion protein using Amylose Magnetic Beads