Amylose Magnetic Beads

Description

An affinity matrix for the small-scale isolation and purification of MBP-fusion proteins. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range. This permits the isolation of MBP-fusion proteins from cell culture supernatants. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that may interact with the immobilized fusion protein.

Supplied as a 10 mg/ml suspension in water containing 20% ethanol.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

Properties and Usage

Storage Temperature

4°C

Binding Capacity

10 ug MBP5* paromysin Δ sal / mg amylose magnetic beads

Storage Notes

  • Do not freeze

Shipping Notes

  • 4°C

Protocols

  1. Isolation of MBP-fusion protein using Amylose Magnetic Beads

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Health Warning

  • Highly flammable liquid and vapor.
  • May cause cancer.