NEBNext^® Ultra^™ II RNA First Strand Synthesis Module

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7771S     -20       NEBNext First Strand Synthesis Reaction Buffer E7421AVIAL -20 1 x 0.192 ml Not Applicable   Random Primers E7422AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext First Strand Synthesis Enzyme Mix E7761AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext Strand Specificity Reagent E7766AVIAL -20 1 x 0.192 ml Not Applicable
E7771L     -20       NEBNext First Strand Synthesis Reaction Buffer E7421AAVIAL -20 1 x 0.768 ml Not Applicable   Random Primers E7422AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext First Strand Synthesis Enzyme Mix E7761AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext Strand Specificity Reagent E7766AAVIAL -20 1 x 0.768 ml Not Applicable

1. Protocol for Directional RNA-seq Workflows and NEBNext^® Ultra^™ II RNA First Strand Synthesis Module (E7771)
2. Protocol for Non-directional RNA-seq Workflows and NEBNext^® Ultra^™ II RNA First Strand Synthesis Module (E7771)
3. Appendix A for use with NEBNext Ultra II RNA First Strand Synthesis Module (E7771)

1. What other materials do I need in addition to the NEBNext Ultra II First Strand Synthesis Module for RNA-seq experiments?
2. What if I have already fragmented my total RNA and need to perform the First Strand Synthesis Reaction?
3. What if I have already fragmented my mRNA and just need to perform the First Strand Synthesis Reaction?

NEBNext Product Citations Search Tool

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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7771S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7771S_L_v1_0021707
• E7771L_v1_10010269
• E7771L_v1_10014366
• E7771S_v1_10009569
• E7771L_v1_10012540
• E7771S_v1_10022793
• E7771S_v1_10012535
• E7771L_v1_10026983
• E7771S_v1_10026978
• E7771L_v1_10040836
• E7771L_v1_10043200
• E7771S_v1_10044266
• E7771L_v1_10047835
• E7771S_v1_10047799
• E7771S_v1_10050670
• E7771L_v1_10039902
• E7771L_v1_10055395
• E7771L_v1_10050458
• E7771S_v1_10050453
• E7771S_v1_10039897
• E7771L_v1_10060358
• E7771L_v1_10065754
• E7771L_v1_10066973
• E7771S_v1_10069645
• E7771L_v1_10070207
• E7771S_v1_10076202
• E7771L_v1_10077654
• E7771L_v1_10061711
• E7771L_v1_10083169
• E7771S_v1_10087239
• E7771L_v1_10071475
• E7771L_v1_10095868
• E7771S_v1_10095910
• E7771L_v1_10100158
• E7771L_v1_10103219
• E7771S_v1_10089912
• E7771L_v1_10089917
• E7771S_v1_10105777
• E7771L_v1_10114867
• E7771L_v1_10121666
• E7771S_v1_10126564
• E7771L_v1_10128588
• E7771S_v1_10133191
• E7771L_v1_10105782
• E7771L_v1_10123370
• E7771S_v1_10142588
• E7771S_v1_10144927
• E7771L_v1_10154876
• E7771S_v1_10123378
• E7771L_v1_10164257
• E7771L_v1_10167103
• E7771S_v1_10146525
• E7771L_v1_10177633
• E7771S_v1_10177635
• E7771S_v1_10165880
• E7771L_v1_10181700
• E7771L_v1_10165873
• E7771L_v1_10195245
• E7771S_v1_10183858
• E7771L_v1_10183848
• E7771L_v1_10199569
• E7771S_v1_10199575
• E7771L_v1_10215775
• E7771S_v1_10204236
• E7771L_v1_10204231
• E7771S_v1_10227658
• E7771L_v1_10227648
• E7771L_v1_10255324
• E7771S_v1_10255246
• E7771S_v1_10260106
• E7771L_v1_10256793

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext First Strand Synthesis Reaction Buffer
• Random Primers
• NEBNext First Strand Synthesis Enzyme Mix
• NEBNext Strand Specificity Reagent

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.