NEBNext® Ultra II RNA First Strand Synthesis Module

Description

The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming.

The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB #E7550) or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111), and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext First Strand Synthesis Reaction Buffer-20
Random Primers-20
NEBNext First Strand Synthesis Enzyme Mix-20
NEBNext Strand Specificity Reagent-20

Properties and Usage

Storage Temperature

-20°C

FAQs

  1. What other materials do I need in addition to the NEBNext Ultra II First Strand Synthesis Module for RNA-seq experiments?
  2. What if I have already fragmented my total RNA and need to perform the First Strand Synthesis Reaction?
  3. What if I have already fragmented my mRNA and just need to perform the First Strand Synthesis Reaction?

Protocols

  1. Protocol for Directional RNA-seq Workflows and NEBNext® Ultra II RNA First Strand Synthesis Module (E7771)
  2. Protocol for Non-directional RNA-seq Workflows and NEBNext® Ultra II RNA First Strand Synthesis Module (E7771)
  3. Appendix A for use with NEBNext Ultra II RNA First Strand Synthesis Module (E7771)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.