The NEBNext® Ultra™ Directional RNA Second Strand Synthesis Module has been
optimized to generate double-stranded cDNA from first-strand cDNA. The
dsDNA generated by the NEBNext Ultra Directional RNA Second Strand Module
can be subsequently converted to blunt-end DNA fragments using the NEBNext
Ultra End Prep Module.
The NEBNext Ultra Directional RNA Second
Strand Synthesis Module is Designed for Use
with the Following:
NEBNext RNA First Strand Synthesis Module (NEB #E7525)
NEBNext Ultra™ End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Ultra™ Ligation Module (NEB #E7445)
NEBNext Singleplex or Multiplex Oligos for Illumina®
(NEB #E7350, #E7335 or #E7500)
USER™ Enzyme (NEB #M5505)
Note: This is included in the NEBNext Oligo sets; however, if you are using alternative oligos,
it is necessary to purchase separately for the directional method.
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a .
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
|Store at (°C)||Concentration
|NEBNext Second Strand Synthesis Enzyme Mix||-20||
|NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix||-20||10X