NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module
The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA. The dsDNA generated by the NEBNext Ultra II Directional RNA Second Strand Module can be subsequently converted to blunt-end DNA fragments using the NEBNext Ultra End Prep Module.
The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
NEBNext RNA First Strand Synthesis Module (NEB #E7525)
NEBNext Ultra™ End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Ultra™ Ligation Module (NEB #E7445)
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335 or #E7500)
USER™ Enzyme (NEB #M5505) Note: This is included in the NEBNext Oligo sets; however, if you are using alternative oligos, it is necessary to purchase separately for the directional method.
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Second Strand Synthesis Enzyme Mix -20 NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix -20 10 X
- Product Categories:
- RNA Library Prep
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.