NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™
The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ includes enzymatic DNA fragmentation in a streamlined, high-performance library prep workflow.
- High yield
- Fast, simple workflow – under 2 hours, with minimal hands-on time
- Compatible with broad range of sample input (10 ng –1 μg)
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The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ includes a master mix of reagents for enzymatic DNA fragmentation combined with end repair. This enables efficient and tunable DNA shearing and blunting with minimized sample loss before performing adaptor ligation and high-fidelity library amplification. The kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time.Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion Xpress™ Barcode Adapters from Thermo Fisher Scientific can be used.
If mechanical shearing of DNA is preferred, a version of the kit without enzymatic fragmentation reagents is available.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact firstname.lastname@example.org for further information.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration Bst 2.0 WarmStart® DNA Polymerase -20 NEBNext DNA Fragmentation Master Mix -20 NEBNext DNA Fragmentation Reaction Buffer -20 10 X T4 DNA Ligase -20 NEBNext DNA Library Adaptors for Ion Torrent -20 NEBNext DNA Library Primers for Ion Torrent™ -20 1 X NEBNext Stop Buffer -20 10 X Magnesium Chloride (MgCl2) (200 mM) -20 T4 DNA Ligase Buffer for Ion Torrent -20 10 X TE Buffer -20 0.1 X NEBNext Q5 Hot Start HiFi PCR Master Mix -20 2 X
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