NEBNext® Fast DNA Library Prep Set for Ion Torrent™
The NEBNext® Fast DNA DNA Library Prep Set for Ion Torrent™ enables library preparation from pre-sheared DNA, with a streamlined, high-performance library prep workflow.
- High yield
- Fast, simple workflow – under 2 hours, with minimal hands-on time
- Compatible with broad range of sample input (10 ng –1 μg)
If enzymatic shearing of DNA is preferred, a version of the kit that includes enzymatic fragmentation reagents is available.
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The NEBNext Fast DNA Library Prep Set for Ion Torrent is suitable when mechanical methods, such as Covaris® acoustic shearing, are preferred for fragmentation of input DNA. The components and protocol for this kit have been optimized for the volumes and concentrations of DNA sheared by mechanical methods, and the kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time..
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext End Repair Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X NEBNext® DNA Library Adaptors for Ion Torrent™ -20 T4 DNA Ligase -20 T4 DNA Ligase Buffer for Ion Torrent™ -20 10 X Bst 2.0 WarmStart® DNA Polymerase -20 NEBNext DNA Library Primers for Ion Torrent™ -20 1 X TE Buffer -20 0.1 X NEBNext® Q5® Hot Start HiFi PCR Master Mix -20
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