The NEBNext® Fast DNA DNA Library Prep Set for Ion Torrent® enables library preparation from pre-sheared DNA, with a streamlined, high-performance library prep workflow.
If enzymatic shearing of DNA is preferred, a version of the kit that includes enzymatic fragmentation reagents is available.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext End Repair Enzyme Mix|
|NEBNext End Repair Reaction Buffer||10 X|
|NEBNext® DNA Library Adaptors for Ion Torrent™||-20|
|T4 DNA Ligase|
|T4 DNA Ligase Buffer for Ion Torrent™||10 X|
|Bst 2.0 WarmStart® DNA Polymerase||-20|
|NEBNext DNA Library Primers for Ion Torrent™||-20||1 X|
|TE Buffer||0.1 X|
|NEBNext Q5 Hot Start HiFi PCR Master Mix||-20||2 X|
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