This kit offers reagent for selectively enriching double-stranded methyl-CpG DNA from fragmented genomic DNA.
MBD-Fc and MCIp were originally developed by Michael Rehli at the University of Regensburg to improve the sensitivity and specificity of conventional CpG binding techniques.
Each kit contains sufficient reagents for the enrichment of methylated DNA from up to 100 μg of fragmented input DNA. If starting with 5 ng to 10 μg of input DNA per experiment, the kit provides sufficient reagents for 25 reactions. Store at 4°C. For long-term storage > 6 months, MBD2-Fc protein should be stored at -20°C.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Bind/Wash Reaction Buffer (5X)||4||5 X|
|NaCl High-Salt Elution Buffer (2M)||4||500 mM|
|MBD2a-Fc Protein||-20||2 mg/ml|
|Fragmented HeLa DNA||4||100 µg/ml|
|LINE Primers for Methylated Controls||4||100 µM|
|RPL30 Primers for Non-methylated Controls||4||100 µM|
|MirA Primers for Input Control||4||100 µM|
|Protein A Magnetic Beads||4||250 µl|
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