The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEB® 5-alpha Competent E. coli (High Efficiency)||-80|
|Q5® Hot Start High-Fidelity 2X Master Mix||-20||2 X|
|SOC Outgrowth Medium||4|
|Control SDM Plasmid||-20||5 µg/ml|
|Control SDM Primer Mix||-20||10 µM|
|KLD Enzyme Mix||-20||10 X|
|KLD Reaction Buffer||-20||2 X|
|pUC19 Vector||-20||0.05 ng/µl|
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