Customers often ask “How can I improve the efficiency of the DpnI digestion?”
The efficiency of the DpnI digestion can be improved by increasing the length of incubation to 30 to 60 minutes at room temperature, or by increasing the temperature to 37 degrees, but only after 5 minutes at room temperature. Finally, you can always add more DpnI.
If you have other questions you can always contact us at info@neb.com.
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