BL21 Competent E. coli

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
C2530H     -80       pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl   SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable   BL21 Competent E. coli C2530HVIAL -80 20 x 0.05 ml Not Applicable

Features

• Routine non-T7 expression

Companion Products

• BL21(DE3) Competent E. coli
• Lemo21(DE3) Competent E. coli
• NiCo21(DE3) Competent E. coli

Materials Sold Separately

• SOC Outgrowth Medium

1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
3. COMPATIBLE EXPRESSION VECTORS/PROMOTERS: P_tac, P_trc, P_lac, PlacUV5, ParaBAD, PrhaBAD, T5-lacO
   Note: Expression from vectors containing a T5-lacO promoter will not be regulated unless the expression vector encodes the lacI gene or a compatible vector expresses LacI or the host strain overproduces LacI (e.g NEB #C3037 NEB Express I^q Competent E. coli)."

1. Transformation Protocol (C2530)
2. 5 Minute Transformation Protocol (C2530)
3. Protocol for Protein Expression Using BL21 (C2530)
4. Transformation Protocol (NEB #C2530)

• Additional E. coli Strain Genotypes
• Competent E. coli Genome Sequences Tool
• Genetic Markers
• McrA, McrBC and EcoKI Strain Phenotypes

• Characteristics of Select E.coli Strains
• Competent Cell Product Comparison
• Competent Cell Selection Guide

1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
2. What are the solutions/recipes (C2530)?
3. What are the strain properties (C2530)?
4. Why are there no colonies or no growth in liquid culture (C2530)?
5. Why is the induced protein insoluble (C2530)?
6. Why is there no protein visible on gel or no activity (C2530)?
7. Can I store competent cells at -20°C instead of -80°C?
8. Which kind of transformation tubes should be used?
9. What volume of DNA can be added into competent cells?
10. What is the shelf life for this strain (NEB #C2350H)?
11. Are NEB's competent cells compatible with the “Mix & Go" protocol?
12. What is the difference between BL21 and BL21(DE3) competent E.coli cells?
13. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?

• Protein Expression & Purification Brochure

• Over 40 years in protein expression and purification – a historical perspective

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• C2530H_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• C2530H_v1_0221608
• C2530H_v1_0231609
• C2530H_v1_0251610
• C2530H_v1_0231610
• C2530H_v1_0261612
• C2530H_v1_0241704
• C2530H_v1_0251707
• C2530H_v1_0271706
• C2530H_v1_0251708
• C2530H_v1_0251710
• C2530H_v1_0261711
• C2530H_v1_0261801
• C2530H_v1_0271803
• C2530H_v1_10011530
• C2530H_v1_10015494
• C2530H_v1_10018939
• C2530H_v1_10021467
• C2530H_v1_10024970
• C2530H_v1_10028042
• C2530H_v1_10032353
• C2530H_v1_10035478
• C2530H_v1_10036757
• C2530H_v1_10039964
• C2530H_v1_10042685
• C2530H_v1_10045312
• C2530H_v1_10048008
• C2530H_v1_10049546
• C2530H_v1_10052488
• C2530H_v1_10056805
• C2530H_v1_10058654
• C2530H_v1_10061186
• C2530H_v1_10063271
• C2530H_v1_10067340
• C2530H_v1_10069841
• C2530H_v1_10074999
• C2530H_v1_10076633
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• C2530H_v1_10082097
• C2530H_v1_10084676
• C2530H_v1_10088587
• C2530H_v1_10093043
• C2530H_v1_10096757
• C2530H_v1_10101759
• C2530H_v1_10106266
• C2530H_v1_10110658
• C2530H_v1_10115834
• C2530H_v1_10119154
• C2530H_v1_10126787
• C2530H_v1_10133313
• C2530H_v1_10139571
• C2530H_v1_10142943
• C2530H_v1_10146577
• C2530H_v1_10152172
• C2530H_v1_10155348
• C2530H_v1_10158010
• C2530H_v1_10162423
• C2530H_v1_10163927
• C2530H_v1_10167743
• C2530H_v1_10172512
• C2530H_v1_10175414
• C2530H_v1_10179233
• C2530H_v1_10183053
• C2530H_v1_10184929
• C2530H_v1_10189557
• C2530H_v1_10193326

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BL21 Competent E. coli
• pUC19 Vector
• SOC Outgrowth Medium

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.