Yeast Carbon Base Medium Powder
The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide.
YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
The following reagents are supplied with this product:
Store at (°C) Concentration Yeast Carbon Base Medium Powder (12 g) 4 Acetamide solution (sterile) (10 ml) 4 100 X
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