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  • T4 Polynucleotide Kinase Reaction Buffer

    Description

    New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    70mM Tris-HCl
    10mM MgCl2
    5mM DTT
    pH 7.6@25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Functional Test (T4 PNK Reaction Buffer):
      The buffer is tested for function by performing a transfer and exchange reaction of radiolabeled phosphate from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is supplied with the T4 Polynucleotide Kinase Reaction Buffer pack?
    2. What is the composition of the T4 Polynucleotide Reaction Buffer?
    3. How should the NEBuffer be used?
    4. The buffer arrived thawed. Are the buffer and enzyme still active?

    Usage Guidelines & Tips

    Troubleshooting Guides