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Monarch kits for plasmid purification are currently available in miniprep format for the purification of up to 20 µg of plasmid DNA from bacterial cultures. Buffers and columns are available separately for added convenience and kits are designed with sustainability in mind  

Reasons to Choose Monarch Kits for Plasmid Purification

SPECIFICATIONS
Culture Volume 1-5 ml, not to exceed 15 OD units
Binding Capacity up to 20 μg
Plasmid Size up to 25 kb
Typical Recovery up to 20 μg. Yield depends on plasmid copy number, host strain, culture volume, and growth conditions.
Elution Volume ≥ 30 μl
Purity A260/280and A260/230≥ 1.8
Protocol Time 10½ minutes of spin and incubation time
Compatible
Downstream
Applications
restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.

 


Unique Column Design

The design of Monarch miniprep columns eliminates buffer retention and salt carryover, resulting in highly pure plasmid DNA for your downstream needs. Elution can be done in as little as 30 µl, and convenient tab and frosted surfaces facilitate handling and labeling.

Monarch Plasmid Miniprep Column Design

 


 

Isolate high-quality, concentrated plasmid DNA

Purify up to 20 µg of plasmid DNA of various sizes with consistently high purity and quality, ready for use in downstream application including digestion, cloning, PCR and transfection of robust cell lines.

Monarch Plasmid Miniprep Kits consistently produce more concentrated plasmid DNA with equivalent yield, purity and functionality as compared to the leading supplier 
Monarch Plasmid Miniprep Kits consistently produce more concentrated plasmid DNA with equivalent yield, purity and functionality as compared to the leading supplier. Preps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel.
Preps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel. 
Plasmid DNA purified using the Monarch Plasmid Miniprep Kit produces transfection efficiencies equivalent to or better than plasmid DNA purified using the Qiagen QIAprep® Spin Miniprep Kit 
Plasmid DNA purified using the Monarch Plasmid Miniprep Kit produces transfection efficiencies equivalent to or better than plasmid DNA purified using the Qiagen QIAprep® Spin Miniprep Kit. Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency.
Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency. 

 


 

Optimized Buffer System

Colored buffers prevent confusion and allow for monitoring of certain steps in the protocol. RNase is provided as part of the neutralization buffer, so there is no need to add it before starting, and no risk of forgetting to add it.

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Less buffer retention as advertised! The indicator system prevents under and over-mixing for lysis and neutralization step, minimizing genomic DNA fragmentation.

– Researcher, University of Massachusetts, Amherst

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