For over 45 years, NEB® has been a world leader in the discovery and production of reagents for the life science industry. Our enzymology expertise effectively positions us to supply reagents for the synthesis of high-quality RNA – from template generation and transcription, to capping, tailing and cleanup after synthesis. These products are designed and manufactured based on decades of molecular biology experience, so that you can be confident they will work for your application.


From research to therapeutic production, NEB’s in vitro transcription portfolio will meet your needs

NEB’s portfolio of research-grade and GMP-grade* reagents enables bench-scale to commercial-scale mRNA manufacturing. Our optimized HiScribe™ kits enable convenient in vitro transcription (IVT) workflows. When it is time to scale up and optimize reaction components, our standalone reagents are readily available in formats matching our GMP-grade offering, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.


Enabling gram-scale RNA synthesis

NEB manufactures and inventories the following enzyme specificities at GMP-grade, meeting customer needs with short lead times:

  • Vaccinia Capping Enzyme – A full system for enzymatic capping based on the Vaccinia virus Capping Enzyme (VCE)

  • T7 RNA Polymerase – RNA Polymerase used for in vitro mRNA synthesis, and is highly specific for the T7 phage promoter

  • COMING SOON, Hi-T7 RNA Polymerase – RNA Polymerase designed for in vitro transcription of RNA at higher temperatures

  • mRNA Cap 2′-O-Methyltransferase – mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA

  • RNase Inhibitor, Murine – RNase Inhibitor, Murine, specifically inhibits RNases A, B and C

  • Pyrophosphatase, Inorganic (E. coli) – Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate

  • DNase I (RNase-free) – DNA-specific endonuclease used for removal of contaminating genomic DNA from RNA samples and degradation of DNA templates in transcription reactions

 

  Not sure whether you need research-grade or GMP-grade materials?
Learn more about the difference between GMP-grade and research-grade reagents 


Template Generation TableCaret_GMPgrade In vitro Transcription TableCaret_GMPgrade RNA Capping TableCaret_GMPgrade Poly(A) Tailing TableCaret_GMPgrade RNAPurification
Q5® High-Fidelity DNA Polymerase HiScribe™ T7 ARCA mRNA Synthesis Kit (with tailing) Monarch® RNA Cleanup Kit (10 μg)
dNTP solution mixes HiScribe T7 ARCA mRNA Synthesis Kit E. coli Poly(A) Polymerase Monarch RNA Cleanup Kit (50 μg)
BspQI, XbaI, HindIII, other restriction enzymes & cloning reagents. Inquire about custom restriction enzyme formulations with Recombinant Albumin. [COMING SOON]
HiScribe T7 High Yield RNA Synthesis Kit
 GMPgrade_icon Vaccinia Capping System GMPgrade_icon   Monarch RNA Cleanup Kit (500 μg)

DNA Assembly:

HiScribe T7 Quick High Yield RNA Synthesis Kit   mRNA Cap 2′-O-Methyltranferase GMPgrade_icon   Lithium Chloride
  HiScribe SP6 High Yield RNA Synthesis Kit   ARCA and other mRNA cap analogs      
  T3 & SP6 RNA Polymerases          
  T7 RNA Polymerase  GMPgrade_icon        
  [COMING SOON]
Hi-T7 RNA Polymerase
 GMPgrade_icon        
  Companion Products         Companion Products
  RNase inhibitor (Murine)  GMPgrade_icon       Monarch RNA Cleanup Binding Buffer
  RNase Inhibitor (Human Placental)         Monarch RNA Cleanup Wash Buffer
  Pyrophosphatase, Inorganic (E. coli)  GMPgrade_icon       Nuclease-free Water
  Pyrophosphatase, Inorganic (Yeast)          
  DNase I (RNase-free)  GMPgrade_icon        
  NTPs          

GMPgrade_icon = available in GMP-grade


Comparability analyses of research-grade and GMP-grade IVT reagents demonstrates qualitative equivalence

Comparability_RNA_GMPgrade

Fluorescent_GMPgradeComparison
Functional expression of active green fluorescent protein in HEK 293
cells after transfection of capped poly-adenylated mRNA demonstrates
qualitative equivalence of GMP-grade and research-grade reagents.

 

"GMP-grade" is a branding term NEB uses to describe reagents manufactured at NEB’s Rowley facility. The Rowley facility was designed to manufacture reagents under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Reagents manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.

This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

 

 

 

 

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