Activity of Restriction Enzymes in PCR Buffers
Enzyme | Taq in std Taq Reaction Buffer | Taq in ThermoPol™ Reaction Buffer | Phusion in Phusion HF Buffer | Q5 in Q5 Buffer* | OneTaq in Standard OneTaq Buffer | LongAmp Taq in LongAmp Taq Buffer |
---|---|---|---|---|---|---|
AatII | <++ | <++ | + | <+ | ++ | + |
Acc65I | +++ | +++ | <+ | <+ | <+ | + |
AccI | ++ | <++ | <+ | <+ | +++ | +++ |
AciI | + | ++ | +++ | ++ | +++ | +++ |
AclI | +++ | +++ | <+ | <+ | +++ | +++ |
AcuI | +++ | +++ | ++ | <+ | +++ | +++ |
AfeI | +++ | +++ | ++ | <+ | +++ | +++ |
AflII | <+ | + | <+ | <+ | + | <+ |
AflIII | <+ | <+ | + | +++ | <+ | <+ |
AgeI-HF® | +++ | +++ | ++ | <+ | +++ | +++ |
AhdI | +++ | <+ | – | – | <+ | <+ |
AleI-v2 | +++ | – | – | – | + | + |
AluI | +++ | +++ | +++ | + | +++ | +++ |
AlwI | <++ | – | <+ | <+ | <+ | <+ |
AlwNI | <++ | <++ | +++ | + | <+ | + |
ApaI | +++ | +++ | <+ | <+ | <+ | – |
ApaLI | +++ | +++ | <+ | <+ | +++ | +++ |
ApeKI @75°C | <++ | <++ | +++ | ++ | <+ | + |
ApoI-HF | +++ | +++ | ++ | + | +++ | +++ |
AscI | +++ | +++ | <+ | <+ | <+ | – |
AseI | + | +++ | + | <+ | ++ | ++ |
AsiSI | +++ | +++ | ++ | <+ | +++ | +++ |
AvaI | +++ | +++ | +++ | <+ | +++ | + |
AvaII | +++ | +++ | ++ | <+ | +++ | +++ |
AvrII | +++ | +++ | <+ | <+ | +++ | +++ |
BaeGI | +++ | +++ | +++ | <+ | +++ | +++ |
BaeI | – | – | ++ | <+ | <+ | <+ |
BamHI§ | +++ | +++ | +++ | <+ | +++ | +++ |
BamHI-HF® | +++ | +++ | – | <+ | <+ | ++ |
BanI | +++ | +++ | +++ | <+ | +++ | +++ |
BanII | +++ | +++ | +++ | <+ | +++ | +++ |
BbsI§ | +++ | +++ | <+ | <+ | +++ | +++ |
BbsI-HF® | – | + | – | – | – | + |
BbvCI | <++ | +++ | – | – | <+ | <+ |
BbvI | +++ | +++ | ++ | <+ | +++ | +++ |
BccI | <+ | <+ | <+ | <+ | <+ | <+ |
BceAI | <+ | <+ | ++ | <+ | + | <+ |
BcgI | +++ | <+ | + | <+ | ++ | ++ |
BciVI | – | – | – | – | <+ | – |
BclI§ | +++ | +++ | +++ | ++ | +++ | +++ |
BclI-HF | +++ | +++ | – | – | + | + |
BcoDI | <+ | <+ | + | <+ | + | <+ |
BfaI | <++ | – | – | <+ | – | – |
BfuAI @50°C | <++ | <++ | + | – | <+ | – |
BglI | <++ | <+ | + | ++ | <+ | <+ |
BglII | <+ | <+ | ++ | + | <+ | <+ |
BlpI | <++ | <++ | <+ | <+ | <+ | – |
BmgBI | <+ | – | + | ++ | <+ | <+ |
BmrI | <++ | <++ | +++ | <+ | +++ | +++ |
BmtI-HF® | ++ | ++ | + | <+ | ++ | +++ |
BpmI | <+ | <+ | +++ | <+ | <++ | <++ |
BpuEI | +++ | +++ | ++ | – | <++ | <++ |
Bpu10I | <+ | <+ | +++ | <+ | ++ | +++ |
BsaAI | ++ | +++ | +++ | ++ | +++ | +++ |
BsaBI @60°C | <+ | + | ++ | <+ | ++ | +++ |
BsaHI | +++ | +++ | +++ | + | +++ | +++ |
BsaI-HF®v2 | + | + | + | <+ | + | ++ |
BsaJI @60°C | +++ | +++ | ++ | <+ | +++ | +++ |
BsaWI @60°C | <++ | <++ | ++ | <+ | + | + |
BsaXI | <++ | <++ | <+ | <+ | <+ | <+ |
BseRI | +++ | +++ | ++ | <+ | ++ | + |
BseYI | +++ | +++ | ++ | ++ | +++ | +++ |
BsgI | <+ | <+ | + | <+ | <+ | <+ |
BsiEI @60°C | +++ | +++ | ++ | <+ | ++ | ++ |
BsiHKAI @65°C | <+ | – | + | ++ | – | – |
BsiWI @55°C§ | ++ | +++ | +++ | <+ | +++ | +++ |
BsiWI-HF® | – | – | – | – | – | – |
BslI | +++ | +++ | +++ | ++ | +++ | +++ |
BsmAI | +++ | +++ | +++ | ++ | <+ | <+ |
BsmBI-v2 @55°C | <+ | <++ | + | ++ | <+ | <+ |
BsmFI | <+ | <+ | ++ | +++ | + | + |
BsmI @65°C | +++ | +++ | <+ | + | +++ | + |
BsoBI | +++ | +++ | +++ | +++ | ++ | +++ |
BspCNI | <+ | <+ | + | <+ | – | – |
BspDI | <++ | <++ | ++ | <+ | +++ | +++ |
BspEI | – | – | <+ | <+ | – | – |
BspHI | <+ | +++ | +++ | <+ | +++ | +++ |
Bsp1286I | + | <+ | <+ | <+ | <+ | <+ |
BspMI | + | +++ | ++ | <+ | <+ | <+ |
BspQI @50°C | <+ | + | +++ | ++ | +++ | +++ |
BsrBI | +++ | +++ | + | <+ | +++ | +++ |
BsrDI | <+ | <+ | + | <+ | <+ | <+ |
BsrFI-v2 | – | <+ | – | – | – | – |
BsrI @65°C | +++ | +++ | +++ | <+ | ++ | +++ |
BssHII | ++ | +++ | + | <+ | +++ | +++ |
BssSI-v2 | +++ | +++ | + | – | +++ | +++ |
BstAPI @60°C | ++ | +++ | ++ | <+ | +++ | +++ |
BstBI @65°C | +++ | +++ | +++ | ++ | +++ | +++ |
BstEII-HF® | + | +++ | <+ | <+ | ++ | ++ |
BstNI @60°C | +++ | +++ | <+ | <+ | <+ | <+ |
BstUI @60°C | +++ | +++ | <+ | <+ | +++ | + |
BstXI | <++ | <++ | + | + | + | <+ |
BstYI @60°C | +++ | +++ | <+ | <+ | ++ | + |
BstZ17I-HF® | +++ | +++ | + | – | +++ | +++ |
Bsu36I | <+ | <+ | <+ | <+ | <+ | + |
BtgI | <+ | +++ | + | <+ | <+ | <+ |
BtgZI @60°C | +++ | +++ | ++ | + | ++ | ++ |
BtsCI @50°C | +++ | +++ | <+ | <+ | +++ | +++ |
BtsIMutI @55°C | + | ++ | – | – | + | + |
BtsI-v2 | – | +++ | + | – | +++ | +++ |
Cac8I | +++ | +++ | <+ | <+ | +++ | ++ |
ClaI | +++ | ++ | <+ | <+ | <+ | ++ |
CspCI | <+ | <+ | + | – | <+ | <+ |
CviKI-1 | – | +++ | ++ | <+ | +++ | +++ |
CviQI @25°C | + | +++ | +++ | + | ++ | +++ |
DdeI | +++ | +++ | + | ++ | +++ | +++ |
DpnI | <+ | +++ | +++ | ++ | ++ | ++ |
DpnII | +++ | +++ | +++ | ++ | +++ | ++ |
DraI | ++ | +++ | +++ | <+ | +++ | +++ |
DraIII-HF® | + | ++ | +++ | ++ | ++ | ++ |
DrdI | ++ | +++ | +++ | <+ | +++ | +++ |
EaeI | +++ | +++ | – | <+ | <+ | <+ |
EagI-HF® | +++ | + | + | <+ | ++ | ++ |
EarI | + | +++ | +++ | <+ | + | <+ |
EciI | <+ | <+ | +++ | ++ | <++ | <++ |
Eco53kI | ++ | +++ | <+ | <+ | +++ | +++ |
EcoNI | <++ | +++ | + | <+ | +++ | +++ |
EcoO109I | +++ | +++ | – | <+ | <+ | + |
EcoP15I | <+ | <+ | + | <+ | <+ | + |
EcoRI§ | + | + | +++ | <+ | – | – |
EcoRI-HF® | +++ | +++ | + | <+ | +++ | +++ |
EcoRV§ | <+ | <+ | + | <+ | – | <+ |
EcoRV-HF® | + | + | <+ | <+ | + | ++ |
Esp3I | +++ | +++ | +++ | – | + | +++ |
FatI @55°C | ++ | ++ | +++ | <+ | <+ | +++ |
FauI @55°C | +++ | + | ++ | <+ | +++ | ++ |
Fnu4HI | +++ | +++ | <+ | <+ | ++ | + |
FokI | +++ | +++ | + | + | +++ | +++ |
FseI | <+ | + | ++ | <+ | +++ | – |
FspI | + | <++ | + | <+ | + | + |
HaeII | +++ | +++ | +++ | <+ | +++ | +++ |
HaeIII | +++ | +++ | +++ | <+ | +++ | +++ |
HgaI | +++ | <+ | + | <+ | <++ | <++ |
HhaI | +++ | +++ | +++ | <+ | +++ | +++ |
HincII | +++ | +++ | <+ | <+ | +++ | +++ |
HindIII§ | ++ | +++ | + | <+ | ++ | +++ |
HindIII-HF® | + | +++ | <+ | <+ | +++ | +++ |
HinfI | +++ | +++ | +++ | +++ | + | +++ |
HinP1I | +++ | +++ | +++ | + | +++ | +++ |
HpaI | +++ | +++ | +++ | <+ | +++ | +++ |
HpaII | + | +++ | <+ | <+ | <+ | <+ |
HphI | +++ | <++ | <+ | <+ | <+ | <+ |
HpyAV | + | +++ | ++ | – | + | ++ |
HpyCH4III | <++ | <++ | + | <+ | <++ | <++ |
HpyCH4IV | ++ | +++ | <+ | <+ | +++ | +++ |
HpyCH4V | +++ | +++ | <+ | <+ | +++ | +++ |
Hpy188I | +++ | +++ | + | <+ | ++ | ++ |
Hpy99I | +++ | +++ | + | – | <+ | <+ |
Hpy166II | + | +++ | ++ | + | +++ | +++ |
Hpy188III | <+ | + | <+ | <+ | + | <+ |
KasI | – | +++ | +++ | <+ | +++ | – |
KpnI-HF® | ++ | ++ | ++ | – | <+ | <+ |
MboI | +++ | +++ | +++ | <+ | +++ | +++ |
MboII | +++ | +++ | ++ | + | + | + |
MfeI-HF® | + | + | – | – | +++ | <+ |
MluCI | +++ | + | <+ | <+ | ++ | + |
MluI-HF® | ++ | ++ | ++ | – | ++ | ++ |
MlyI | <+ | +++ | ++ | + | <+ | + |
MmeI | <+ | <+ | ++ | – | <+ | <+ |
MnlI | +++ | +++ | + | + | + | + |
MscI | <+ | <+ | + | <+ | <+ | <+ |
MseI | + | <+ | <+ | <+ | <+ | <+ |
MslI | +++ | +++ | + | <+ | +++ | ++ |
MspA1I | +++ | +++ | +++ | <+ | ++ | +++ |
MspI | +++ | +++ | +++ | <+ | ++ | +++ |
MwoI @60°C | +++ | +++ | +++ | +++ | ++ | +++ |
NaeI | <+ | <+ | + | <+ | <+ | <+ |
NarI | – | – | ++ | <+ | +++ | +++ |
NciI | +++ | +++ | <+ | <+ | + | <+ |
NcoI§ | +++ | +++ | + | <+ | ++ | ++ |
NcoI-HF® | +++ | +++ | – | <+ | ++ | + |
NdeI | ++ | <++ | +++ | ++ | ++ | <+ |
NgoMIV | – | – | + | <+ | <+ | <+ |
NheI-HF® | +++ | +++ | – | <+ | ++ | ++ |
NlaIII | +++ | <+ | + | <+ | ++ | <+ |
NlaIV | <+ | +++ | +++ | <+ | +++ | +++ |
NmeAIII | <+ | <+ | +++ | – | <+ | <+ |
NotI§ | +++ | ++ | + | <+ | <+ | <+ |
NotI-HF® | ++ | +++ | <+ | <+ | <+ | + |
NruI-HF® | +++ | ++ | – | – | + | – |
NsiI§ | +++ | +++ | +++ | + | ++ | + |
NsiI-HF® | +++ | +++ | +++ | ++ | +++ | +++ |
NspI | +++ | +++ | <+ | <+ | +++ | ++ |
PacI | +++ | +++ | <+ | <+ | ++ | +++ |
PaeR7I | +++ | +++ | <+ | <+ | +++ | +++ |
PaqCI | + | – | – | – | +++ | ++ |
PciI | + | <+ | – | <+ | – | – |
PflFI | +++ | +++ | <+ | <+ | <+ | + |
PflMI | + | + | +++ | <+ | ++ | +++ |
PleI | + | +++ | <+ | <+ | <+ | <+ |
PluTI | +++ | +++ | + | <+ | +++ | +++ |
PmeI | +++ | +++ | <+ | <+ | +++ | +++ |
PmlI | + | – | – | – | + | <+ |
PpuMI | +++ | +++ | +++ | <+ | +++ | +++ |
PshAI | +++ | +++ | <+ | <+ | <+ | <+ |
PsiI-v2 | + | +++ | – | – | +++ | +++ |
PspGI @75°C | +++ | +++ | +++ | +++ | +++ | +++ |
PspOMI | +++ | +++ | + | <+ | +++ | +++ |
PspXI | +++ | +++ | ++ | <+ | +++ | +++ |
PstI§ | ++ | ++ | + | + | <+ | <+ |
PstI-HF® | ++ | +++ | ++ | <+ | ++ | + |
PvuI-HF® | +++ | +++ | +++ | <+ | ++ | +++ |
PvuII§ | +++ | +++ | + | <+ | +++ | +++ |
PvuII-HF® | ++ | + | – | – | <+ | <+ |
RsaI | +++ | +++ | ++ | <+ | +++ | +++ |
RsrII | <++ | <++ | – | – | <+ | <+ |
SacI-HF® | +++ | +++ | <+ | <+ | <+ | ++ |
SacII | +++ | +++ | +++ | <+ | ++ | + |
SalI§ | <+ | <+ | ++ | + | – | – |
SalI-HF® | +++ | + | +++ | <+ | + | +++ |
SapI | <++ | <++ | ++ | <+ | ++ | ++ |
Sau3AI | +++ | +++ | <+ | <+ | <+ | <+ |
Sau96I | <++ | <++ | + | + | +++ | +++ |
SbfI-HF® | + | + | – | – | <+ | <+ |
ScaI-HF® | + | + | <+ | <+ | – | – |
ScrFI | +++ | +++ | +++ | +++ | +++ | +++ |
SexAI | +++ | +++ | +++ | <+ | +++ | +++ |
SfaNI | – | – | ++ | <+ | <++ | <++ |
SfcI | +++ | +++ | <+ | <+ | + | + |
SfiI @50°C | <+ | +++ | – | – | +++ | +++ |
SfoI | +++ | +++ | +++ | <+ | + | +++ |
SgrAI | <++ | <++ | ++ | <+ | + | +++ |
SmaI | <+ | +++ | ++ | <+ | +++ | +++ |
SmlI @55°C | <+ | <+ | + | <+ | + | + |
SnaBI | +++ | <+ | <+ | <+ | +++ | +++ |
SpeI-HF® | +++ | +++ | <+ | – | +++ | +++ |
SphI§ | +++ | +++ | ++ | + | <+ | <+ |
SphI-HF® | +++ | +++ | + | <+ | +++ | +++ |
SrfI | + | <+ | +++ | <+ | + | ++ |
SspI-HF® | ++ | ++ | + | <+ | +++ | +++ |
StuI | +++ | +++ | <+ | <+ | +++ | +++ |
StyD4I | <++ | <++ | + | <+ | <+ | <+ |
StyI-HF® | ++ | + | <+ | <+ | ++ | +++ |
SwaI @25°C | <+ | <+ | <+ | <+ | <+ | +++ |
TaqI-v2 @65°C | +++ | +++ | + | <+ | +++ | +++ |
TfiI @65°C | <++ | <++ | <+ | <+ | ++ | ++ |
TseI @65°C | +++ | +++ | +++ | +++ | +++ | +++ |
Tsp45I @65°C | <+ | +++ | – | – | + | <+ |
TspMI @75°C | +++ | +++ | + | <+ | +++ | +++ |
TspRI @65°C | + | + | <+ | <+ | +++ | +++ |
Tth111I @65°C | +++ | +++ | ++ | <+ | <+ | + |
XbaI | +++ | +++ | <+ | – | ++ | ++ |
XcmI | +++ | +++ | + | <+ | +++ | +++ |
XhoI | <+ | <+ | +++ | <+ | ++ | +++ |
XmaI | +++ | +++ | + | <+ | – | – |
XmnI | +++ | +++ | <+ | <+ | ++ | +++ |
ZraI | +++ | +++ | <+ | <+ | ++ | + |
§ An HF version of this enzyme is available.
Phusion® was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
Phusion® is a registered trademark of Thermo Fisher Scientific.
ThermoPol™ is a trademark of New England Biolabs, Inc.