Histone H3.1 Human, Recombinant

1. When running SDS-PAGE gels, Histone H3.1 can self-oligomerize if freshly prepared DTT (dithiothreitol) is not used when preparing the sample for gel electrophoresis.
2. 1 mg/ml, 65 µM is calculated using the molar extinction coefficient for Histone H3.1 (4080) and its absorbance at 280 nm (3,4). 1.0 A280 units = 3.8 mg/ml

1. Kornberg, R.D. (1977). Annu. Rev. Biochem. 46, 931-954.
2. van Holde, K.E. (1989). Chromatin. 1-497.
3. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem.. 182, 319-326.
4. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
5. Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.

1. Do the histones need to be reconstituted?
2. What are the recommended histone storage conditions?
3. Are the histones fusion proteins or tagged proteins?
4. Can the histones be used as substrates for protein modification enzymes? Which ones?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M2503S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M2503S_v1_0051708
• M2503S_v1_0051712
• M2503S_v1_10018475
• M2503S_v1_10034969
• M2503S_v1_10049210
• M2503S_v1_10067282
• M2503S_v1_10086522
• M2503S_v2_10128924
• M2503S_v2_10163378
• M2503S_v2_10178816
• M2503S_v2_10208777
• M2503S_v1_0051804

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Histone H3.1 Human, Recombinant

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