FAQ: Can the histones be used as substrates for protein modification enzymes? Which ones?

They would presumably serve as excellent substrates for any histone modification enzyme. We ensure that the recombinant histones are not modified at any potential modification site by producing them in E.coli and then further checking each histone’s mass by ESI-TOF MS (Electrospray Ionization-Time of Flight Mass Spectrometry).

The histone modification enzymes that we have tested are: