Home DNA Modifying Enzymes and Cloning Technologies T4 Gene 32 Protein

T4 Gene 32 Protein
NEB4 Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 65°

Available in Glycerol-free format. Please inquire.

T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair.

  • Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure
  • Increase yield and processivity of reverse transcriptase during RT-PCR
  • Increase yield and specificity of PCR products from soil samples
  • Used in Recombinase Polymerase Amplification (RPA)
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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M0300S 10 mg/ml 100 µg $95.00 $95.00
M0300L 10 mg/ml 500 µg $387.00 $387.00
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  • T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair (1-2). It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication (3). It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures (4-5). Recently, it has been shown to improve restriction enzyme digestion (6), improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR (7-9), enhance T4 DNA polymerase activity (10-11), as well as increase the yield of PCR products (12).

    Product Source

    An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage
    This product is related to the following categories:
    ssDNA Binding Proteins
    This product can be used in the following applications:
    Recombinase Polymerase Amplification and SIBA,
    RT-PCR & cDNA Synthesis

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Reaction Conditions

      1X NEBuffer™ 4
      Incubate at 37°C

      1X NEBuffer™ 4
      50 mM Potassium Acetate
      20 mM Tris-acetate
      10 mM Magnesium Acetate
      1 mM DTT
      (pH 7.9 @ 25°C)

      Storage Buffer

      20 mM Tris-HCl
      100 mM NaCl
      0.5 mM DTT
      1 mM EDTA
      50% Glycerol
      pH 8 @ 25°C

      Heat Inactivation

      65°C for 20 minutes

      Molecular Weight

      Theoretical: 33506 daltons

    • Advantages and Features

      Application Features

      • Stabilization and marking of ssDNA structures (4-5)
      • Increase yield and processivity of RT during RT-PCR (7-9)
      • Increase yield and specificity of PCR products from soil samples (10-12)

    • Related Products

      Materials Sold Separately

    • References

      1. Alberts, B. and Frey, L. (1970). Nature. 227, 1313-1318.
      2. Huberman, J.A. and Kornberg, A. (1971). J. Mol. Biol. 62, 39-52.
      3. Topal, M.D. and Sinha, N.K. (1983). J. Biol. Chem. 258, 12274-12279.
      4. Schwartz, K. et al. (1990). Nucl. Acids Res. 18, 1079.
      5. Kornberg, A. and Baker, T. DNA Replication. (2nd ed.), 328-332.
      6. Bittner, M. et al. (1979). J. Biol. Chem. 254, 9565-9572.
      7. Delius, H. et al. (1972). J. Mol. Biol.. 67, 341-350.
      8. Wu, M. and Davidson, N. (1975). Proc. Natl. Acad. Sci. USA. 72, 4506.
      9. Dombroski, D.F. and Morgan, A.R. (1985). J. Biol. Chem. 260, 415-447.
      10. Villalva, C. et al. (2001). Biotechniques. 31, 81-86.
      11. Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
      12. Jeffries, D. and Farquharson, C. (2002). Anal Biochem. 308, 192-194.

  • Quality, Safety & Legal

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