For improved performance with BA.2 variants, spike-in primers can now be provided, for use in combination with VarSkip Short v2 primers. Please see this FAQ for more information, including primer sequences.
See below, including Figure 1 in the Product Information section, for information on VarSkip Short v2 performance with spike-in primers. Information on primer variant overlaps can be found here.
A bulk format (2,304 reactions) is also available. Please contact us for more information.
The NEBNext® ARTIC products were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These are based on the original work of the ARTIC Network(1) to develop a multiplexed amplicon-based whole-viral-genome sequencing approach.
NEBNext ARTIC SARS-CoV-2 RT-PCR Module contains the reagents required for cDNA synthesis and targeted cDNA amplification from SARS-CoV-2 genomic RNA.
This kit includes two options for primers, including new VarSkip Short v2 primers:
Full kits that also include library prep reagents optimized for the ARTIC workflow are available, for smaller (~150 bp) (NEB #E7658) or larger (~400 bp) (NEB #E7650) insert libraries for Illumina® sequencing, and for Oxford Nanopore Technologies® sequencing (NEB #E7660). Note that E7650 does not include VarSkip v2 primers.
1. (Josh Quick 2020. nCoV-2019 sequencing protocol v2 (GunIt). protocols.io https://dx.doi.org/10.17504/protocols.io.bdp7i5rn).
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