Information on primer overlaps with Omicron and Delta variants.
This kit does not include VarSkip primers. For improved performance with variants, VarSkip Short primers are included in the NEBNext ARTIC SARS-CoV-2 kits for smaller (~150bp) insert libraries for Illumina sequencing (NEB #E7658) and for Oxford Nanopore Technologies® sequencing (NEB #E7660), as well as in the NEBNext ARTIC SARS-CoV-2 RT-PCR Module (NEB #E7626).
A bulk format (2,304 reactions) is also available. Please contact us for more information.
The NEBNext® ARTIC kits were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network (1). The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach.
NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina® and Oxford Nanopore Technologies® sequencing. The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range.
If shorter inserts are preferred, the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658) generates libraries with ~150 bp inserts, compatible with 2 x 75 bp sequencing.
1. (Josh Quick 2020. nCoV-2019 sequencing protocol v2 (GunIt). protocols.io https://dx.doi.org/10.17504/protocols.io.bdp7i5rn).
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