K. lactis GG799 Competent Cells

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
C1001S     -80       K. lactis GG799 Competent Cells C1001SVIAL -80 5 x 0.2 ml Not Applicable   NEB® Yeast Transformation Reagent (5 ml) M2570SVIAL 4 1 x 5 ml Not Applicable

Companion Products

• Yeast Carbon Base Medium Powder

1. Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.
2. Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.
3. Plates containing colonies can be stored at 4°C for up to two weeks.

1. Transformation Protocol for K. lactis GG799 Competent Cells (C1001)

• Improved method for assembly of linear yeast expression C1001
• Co-expression of Multiple Proteins in Kluyveromyces lactis

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• C1001S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• C1001S_v0_10008774
• C1001S_v1_10007435
• C1001S_v1_10023735
• C1001S_v1_10032858
• C1001S_v1_10041172
• C1001S_v1_10048296
• C1001S_v1_10058974
• C1001S_v1_10062305
• C1001S_v1_10064459
• C1001S_v1_10075397
• C1001S_v1_10082855
• C1001S_v1_10085966
• C1001S_v1_10101277
• C1001S_v1_10111306
• C1001S_v1_10123684
• C1001S_v1_10127457
• C1001S_v1_10148415
• C1001S_v1_10164855
• C1001S_v1_10173973
• C1001S_v1_10190539
• C1001S_v1_10200464
• C1001S_v1_10211478
• C1001S_v1_10226373
• C1001S_v1_10251811

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• K. lactis GG799 Competent Cells
• NEB^® Yeast Transformation Reagent (5 ml)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.