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Home Discontinued Products NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

This product has been replaced with NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™) (NEB #B7030).

  • Catalog # B7200 was discontinued on December 15, 2021
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. 

Set contains 1.25ml vial of each buffer.
 

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  • Product Information
    New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
     
    NEBuffer 1.1
    1X Buffer Components
    10mM Bis-Tris-Propane-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.0@25°C

    NEBuffer 2.1
    1X Buffer Components
    50mM NaCl
    10mM Tris-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.9@25°C

    NEBuffer 3.1
    1X Buffer Components
    100mM NaCl
    50mM Tris-HCl
    10mM MgCl2
    100μg/ml BSA
    pH 7.9@25°C

    CutSmart Buffer
    1X Buffer Components
    50mM Potassium Acetate
    20mM Tris-acetate
    10mM Magnesium Acetate
    100μg/ml BSA
    pH 7.9@25°C
    This product is related to the following categories:
    Discontinued (<3 years)
  • Citations & Technical Literature
  • Quality, Safety & Legal
    • Quality Control Assays
      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    • Specifications & Change Notifications

      Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
    • Certificate of Analysis
      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
    • Legal and Disclaimers
      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

      Trademarks

      New England Biolabs® is a registered trademark of New England Biolabs, Inc.
      CUTSMART™ is a trademark of New England Biolabs, Inc.

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