PURExpress
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- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Protein Expression & Purification Brochure
- PURExpress® vs. NEBExpress® Application Chart
- Protein Expression and Purification Selection Chart
Feature Articles
Brochures
Selection Tools
- His-Tagged Translation Factors
- Initiation Factors (IF1, IF2, IF3)
- Elongation Factors (EF-Tu, EF-Ts, EF-G)
- Release Factors (RF1, RF2, RF3)
- Ribosome Recycling Factor
- 20 Aminoacyl tRNA synthetases
- Methionyl tRNA formyltransferase
- E. coli Ribosomes
- E. coli tRNAs
- Energy Regeneration System
- NTPs, Amino Acids, Salts, Buffer
In addition, recombinant T7 RNA polymerase is used to couple transcription to translation. The PURE system represents an important step towards a totally defined in vitro transcription/translation system, thus avoiding the “black box” nature of the cell extract-based systems.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.