The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
- Synthesize high yields of protein (typically 0.5 mg/ml)
- Protein can be synthesized and visualized in approximately 2 - 4 hours
- Synthesize various target proteins ranging from 17 to 230 kDa
- Templates can be plasmid DNA, linear DNA or mRNA
- Minimal protease activity ensures stability of desired target protein
- RNase contamination can be inhibited by the supplied RNase inhibitor, eliminating clean-up steps
- Protein can be isolated with affinity purification or subject to direct functional analysis
- Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
- Flexible reaction conditions achieve maximum yield; protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
- Reactions can be miniaturized or scaled up
- Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820) and NEBExpress GamS Nuclease Inhibitor (NEB #P0774)
- What is the difference between NEBExpress® Cell-free E. coli Protein Synthesis System and the PURExpress In Vitro Protein Synthesis Kit?
- Are the yields with NEBExpress® Cell-free E. coli Protein Synthesis System comparable with the PURExpress In Vitro Protein Synthesis Kit?
- Based on my application, should I use the NEBExpress® Cell-free E. coli Protein Synthesis System (NEB #E5360) or the PURExpress® In Vitro Protein Synthesis Kit (NEB #E6800)?
The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Protein Expression & Purification Brochure
- Purification Beads, Columns and Resins Brochure
- PURExpress® vs. NEBExpress® Application Chart
- Protein Expression and Purification Selection Chart
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Watch this tutorial explaining the streamlined workflow for our new NEBExpress® Cell-free Protein Synthesis System to learn how you can easily synthesize your protein in as little as 2 to 4 hours.
This webinar discusses the fundamental workflows and advantages of cell-free (in vitro) protein synthesis versus traditional cellular expression methods.