RT-PCR

LunaScript Multiplex One-Step RT-PCR Kit

The LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555) offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. The 5X reaction mix contains a reaction buffer supplemented with dNTPs and is optimized for robust multiple target detection in a simple workflow. The 25X enzyme mix features Luna^® WarmStart^® Reverse Transcriptase and Q5^® Hot Start High-Fidelity DNA Polymerase.

OneTaq^® One-Step RT-PCR Kit

The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase.

OneTaq^® RT-PCR Kit

The OneTaq^® RT-PCR Kit (NEB #E5310) combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions.

LunaScript^®, Warmstart^®, Q5^®, ProtoScript^®, LongAmp^®, and OneTaq^® are registered trademarks of New England Biolabs, Inc.