First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)

Mix the following components and incubate at 42°C for 1 hour.
Template RNA up to 1 µg*
d(T)23VN (50 µM) or Random Primer Mix (60 µM) 2 µl
10X AMV buffer 2 µl
10 mM dNTP Mix  1 µl
RNase Inhibitor (40 U/µl) 0.2 µl
Nuclease-free H2O To a total volume of 20 µl

* 1 ng-1 µg total RNA or 50 pg-100 ng poly(A)-RNA