α1-2,3,4,6 Fucosidase

Companion Products

• α1-2 Fucosidase

1. 8 units of enzyme wereinactivated by incubation at 100°C for 10 minutes.

1. Vainauskas, S., New England Biolabs, Inc. Unpublished observation
2. Wong-Madden, S.T. and Landry, D. (1995). Glycobiology 5. 19-28.

1. Typical Reaction Conditions for α1-2, 3, 4, 6 Fucosidase (P0748)

• Glycan Analyzer

1. What are Glycosidases and their uses?
2. Does this enzyme require denaturing conditions to act on glycoproteins?
3. What is a good method to re-purify a glycan or glycopeptide after exoglycosidase treatment?
4. Do detergents inhibit glycosidases?
5. What is the difference between α1-2 Fucosidase, α1-3,4,6 Fucosidase and α1-3,4 Fucosidase?
6. Can I use α1-3,4 Fucosidase in a double digest with other exoglycosidases and/or endoglycosidases?
7. How much exoglycosidase should be used?
8. Can α1-3,4 Fucosidase and α1-2,3,4,6 Fucosidase cleave branched and complex substrates?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

• Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. Anal Chem.. PubMedID: 27033327
• O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris (2017) Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex J Proteome Res; 16, 4237-4243.

• Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
• Redesigning glycosidase manufacturing quality for pharmaceutical and clinical applications

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0748S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0748S_L_v1_0021603
• P0748S_L_v2_0021609
• P0748S_L_v2_0021702
• P0748S_L_v2_0021707
• P0748S_L_v2_0021711
• P0748S_L_v2_0031801
• P0748S_v2_10014924
• P0748L_v2_10014926
• P0748S_v2_10030272
• P0748L_v2_10030274
• P0748L_v2_10044936
• P0748S_v2_10044934
• P0748S_v2_10055869
• P0748L_v2_10055872
• P0748S_v2_10061241
• P0748L_v2_10065555
• P0748S_v2_10065556
• P0748L_v2_10070026
• P0748S_v2_10082487
• P0748L_v2_10092713
• P0748S_v2_10092716
• P0748L_v2_10099072
• P0748S_v2_10099075

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• α1-2,3,4,6 Fucosidase

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.