FAQ: Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?

RNA will bind to the Monarch DNA Cleanup Column, but the buffers and protocol have not been optimized to ensure reliable, RNase-free recovery. Therefore, we do not recommend use of this kit to purify RNA. NEB offers 3 kits designed specifically for the cleanup and concentration of RNA samples (NEB #T2030, #T2040, #T2050).