Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Histone H3.1 is an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing.
Highly pure, recombinant protein
Mass confirmed by ESI-TOF MS
Tested for exonuclease, endonuclease and protease contamination
Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.
Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing.
When running SDS-PAGE gels, Histone H3.1 can self-oligomerize if freshly prepared DTT (dithiothreitol) is not used when preparing the sample for gel electrophoresis.
1 mg/ml, 65 µM is calculated using the molar extinction coefficient for Histone H3.1 (4080) and its absorbance at 280 nm (3,4). 1.0 A280 units = 3.8 mg/ml
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