Histone H1⁰ Human, Recombinant

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
NEB # Stored at (°C) M2501S -20 Component Name Component # Stored at (°C) Amount Concentration Histone H10 Human, Recombinant M2501SVIAL -20 1 x 0.1 ml 1 mg/ml

1. 1 mg/ml, 48 µM iscalculated using the molar extinction coefficient for Histone H1 (3840) and itsabsorbance at 280 nm (4,5).  1.0 A₂₈₀ units = 5.4mg/ml.
2. There is a small percentage of Histone H1⁰ with a mass of 20863.27 which is a +131 Da difference from the major species. This correlates to Histone H1⁰ with an intact N-terminal methionine (6).
3. Synonyms: Histone H1.0, Histone H1(0), Histone H1'

1. van Holde, K.E. (1989). Chromatin. 1-497,
2. Marzluff, W.F., et al. (2002). Genomics. 80, 487-497.
3. Pehrson, J.R. and Cole, R.D. (1982). Biochem. 21, 456-460.
4. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. . 182, 319-326.
5. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
6. Qing, X. et al. (2010). Biochemistry. 49, 5588-5599.

1. Do the histones need to be reconstituted?
2. What are the recommended histone storage conditions?
3. Are the histones fusion proteins or tagged proteins?
4. Can the histones be used as substrates for protein modification enzymes? Which ones?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M2501S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M2501S_v1_0061708
• M2501S_v1_0061804
• M2501S_v1_10028263
• M2501S_v1_10048766
• M2501S_v1_10053619
• M2501S_v1_10060663
• M2501S_v1_10061893
• M2501S_v1_10073646
• M2501S_v1_10075399
• M2501S_v2_10109982
• M2501S_v2_10140380
• M2501S_v2_10157554
• M2501S_v2_10208723

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Histone H1⁰ Human, Recombinant

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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