NEBNext^® Primers for Epigenetics (Unique Dual Index Set 2B)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E3392S     -20       NEBNext® Primers for Epigenetics (Unique Dual Index Set 2B) Plate E3399AVIAL -20 1 x 5 μl/well 10 µM

Companion Products

• NEBNext^® Enzymatic 5hmC-seq Kit
• NEBNext^® Primers for Epigenetics (Unique Dual Index Set 3)

1. Where can I find protocols for use of the NEBNext^® Primers for Epigenetics (Unique Dual Index Set 2B), including index pooling guidelines? (NEB #E3392)

• Forward Strand Workflow Sample Sheet for E3392: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
• NEB_E3392_UDI_IlluminaLRM
• Reverse Complement Workflow Sample Sheet for E3392: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

• NEBNext Selector

1. Are Sample Sheets available for use with the NEBNext® Primers for Epigenetics?
2. Are dual-indexed libraries compatible with single end sequencing?
3. What is provided in the NEBNext^® Primers for Epigenetics?
4. Can the NEBNext^® Primers for Epigenetics be used to address “index hopping” with Illumina^® patterned flow cells?
5. How many indices are available with the 24 reaction or 96 reaction size of the NEBNext^® Primers for Epigenetics?
6. Are the NEBNext^® Primers for Epigenetics the same as NEBNext^® Multiplex Oligos for Illumina^®?
7. Are the NEBNext^® Primers for Epigenetics validated for the E5hmC-seq™ workflow?
8. What is the concentration of the Index Primers in NEBNext^® Primers for Epigenetics?
9. How many reactions worth of primer are in each well for the NEBNext^® Primers for Epigenetics?
10. Can I use the 96-well primer plate supplied with the NEBNext^® Primers for Epigenetics to do my PCR reactions?
11. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext^® Primers for Epigenetics on Illumina^® sequencing instruments?
12. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
13. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E3392S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E3392S_v1_10231036

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.