FAQ: What other materials do I need in addition to the NEBNext Ultra II First Strand Synthesis Module for RNA-seq experiments?
For strand-specific RNA-seq workflows, use the following kits:
NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB #E7550)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
NEBNext Ultra II Ligation Module (NEB #E7595)
NEBNext Ultra II Q5 Master Mix (NEB #M0544)
For non-directional RNA-seq workflows, use the following kits:
NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
NEBNext Ultra II Ligation Module (NEB #E7595)
NEBNext Ultra II Q5 Master Mix (NEB #M0544)
For adaptors and primers, NEBNext Oligos are compatible, as are adaptors and primers from other sources.